Study Of The Radio-sensitization Effect Of Plumabgin In Tongue Cancer Via ATM/NF-?B Signaling Pathway

Objective:To study radiation sensitizing effect and mechanism of Plumbagin on human tongue squamous carcinoma line SCC9.Methods: 1.The SCC9 cells were cultured in vitro, which were radiated by clinical linear accelerator in different doses, and continued to culture different times. 2.At last flow cytometry assay was applied to test cell cycle of carcinoma cells. The purpose is that choosing optimum time and radiation dose. 3.MTT assay was employed to examine the growth status of the cells and determine the optimum dose for research. 4.Cells were divided into blank group, plumbagin group, radiation group, united group(Plumbagin and X-ray), At the same time,we needed set up parallel for putting in ATM inhibitors KU-55933 to compare with before. 5.The flow cytometry assay was used to detect the apoptosis ratio of SCC9 cells in each group.6.Western Blot was detected the expression of ATM?p-ATM?NF-?B.Results:1. The result of flow cytometry assay is that optimum dose of radiation is 4Gy, and incubat 24 hour after radiation as the experimental conditions. 2. The optimum dose of plumbagin on SCC9 cells is 1.34?m/L for 24 h.3. PLB could inhibit the expression of p-ATM?NF-?B,which were activated by IR.4. The group of PLB+IR can enhance the apoptosis of SCC9 cells high to 45.4 percent.5. the expression of NF-?B in each group had no significant change when the expression of p-ATM were inhibited by KU-55933(p>0.05).Conclusion:Plumbagin has radio-sensitizing effect on tongue cancer SCC9 cells, which was achieved by inhibiting the ATM / NF-?B signaling pathway.