The Effect On Taurine Through PI3K/AKT Which Is Applied To PASMCs Of Hypoxia Rat

Objective:This article investigated the effect of Tau on PTEN protein expression,and investigate Tau whether through P13K/AKT signal pathway to inhibit the proliferation of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs).Methods:The original generation develop PASMCs in rats,uesd 2-5 generations in experiments.The concentration of Tau is 80 mmol/L. The concentration of LY294002 is 10 mmol/L.The concentration of Rapamycin is 10 mmol/L. ? Using MTT to determate the relationship of dose conversion and function.Western blot and immunofluorescence assay determate the effect of Tau on PTEN protein expression. The PASMCs were divided into 4 groups:normoxia group,normoxia+Tau group,hypoxia group,hypoxia+Tau group. ? Using westernblot and immunofluorescence and flow cytome-try(FCM) assay the effect of P13K/AKT signal pathway. The PASMCs were divided into 5 groups:normoxia group.hypoxia group,hypoxia+Tau group, hypoxia+Rapamycin group, hypoxia+Tau+Rapamycin group and normoxia group,hypoxia group,hypoxia+Tau group, hypoxia+LY294002 group, hypoxia+Tau +LY294002 group.Results:?MTT assay revealed that taurine(80 mmol/L) significantly inhibited PASMCs proliferation induced by hypoxia(p<0.05),but had no effect on PASMCs under normoxia condition. ?Tau could increase the expression of PTEN by hypoxia. ? The expression of PCNA(p<0.01) and CyclinD (p<0.05) in hypoxia group were higher than that in normoxia group,which were significantly suppressed by taurine. There was no effect of Tau on the expression of P-AKT?AKT?mTOR.? Tau had no effects on the expresstion of P-AKT(p<0.01) and mTOR(p<0.01) under normoxia condition,however,Tau decreased the expresstion of P-AKT and mTOR induced by hypoxia. Compared with hypoxia group,both LY294002 (10 ?mol/L) and Tau (80 mmol/L) could decrease the expression of PCNA, CyclinD?P-AKT. In Tau+LY294002 group,the expression of PCNA,CyclinD and P-AKT were decreased much more than simply used Tau or LY294002. ?Flow cytometry results confirmed that, compared with the control group, Tau and LY294002 can prevent the hypoxia status of PASMCs into the S phase, so as to inhibit its proliferation.Conclusion:Tau could increase the expression of PTEN by hypoxia.Tau also could inhibit PASMCs proliferation induced by hypoxia and this maybe via P13K/AKT pathway.