| Objective:Use freshly extracted, single-rooted premolars samples to make Candida albicans infected models. Chose Cinnamic aldehyde and 1mg/ml Paeonol as root canal disinfection test drugs, using Formocresol of root canal disinfection drugs usually used in clinical as the controls, observe the sterilizing effect to Candida albicans of the two kinds of Chinese medicine, in order to provide a reference for clinical application.Methods:60 freshly extracted, single-rooted premolars were randomly divided into five groups. There were 12 specimens in each group. Separate the teeth into two parts at cervix dentis that is 12mm to the apex. After gaining access, the pulp was removed. The teeth were manually instrumented with Protaper up to size 25# using a passive step-back technique. Each root canal preparation process replace two devices, fitted with 5ml 2.5% NaClO solution and 5ml 17% MTAD with a 23G lateral opening needle, to wash the canal.After preparation, giving the canal a quick and high doses rinse with a device fitted with 5ml 0.9% NaCl, then wash them with ultrasonic plaster, and dry the canal. Blocking the apical of the teeth 2mm to the apex by 3M P60. All roots were put into Sabouraud’s medium, then incubated at 37℃(5% C02).24h later, through the observation to turbidity of Sabouraud’s medium to make sure the complete sterilization. Recovered and identificated Candida albicans. And inoculated in Sabouraud’s medium, then adjust turbidity into 0.5M with turbidimetry.In addition to the negative control group,60 specimens were placed in standard broth and standing at 5% CO2 electric incubator (37 ℃) within 7 days. Take out the specimens and identified bacterial species at the 7th day, make sure there is no other kind of bacteria mixed in 48 specimens were infected with Candida albicans. Using different sterile twisted cotton with cinnamic aldehyde, paeonol, Formocresol, and NaCl (about 0.2ul) into the canal of each group of specimens. Temporary blocking the apical of the teeth with Caxiton.The negative control group was put in the NaCl solution. Fix the tooth apex of specimens which is sealed with paraffin on the Petri dish, and standing at 5% CO2 electric incubator (37 ℃), respectively, in 3d and 7 th after each set of samples taken 6 to remove the temporary blocking agent and the twisted cotton, wash the canal with distilled water till there is no drug residues. Shavings the dentin with 25# H file to get the powder sample and make germiculture and bacterium colony computation.At the same time, take fast Gram’s staining and bacterial species identification of BHI broth in vitro under biological microscope, make sure there is no other kind of bacteria within.Results:1 The counting results of cultured bacteria before root canal sealing agents showed that the number of fungal in root canal of each group has no statistical significance befor drug administration (P> 0.05).2 In the 3rd day of root canal sealing agents, the fungal number in root canal of Cinnamic aldehyde and Formocresol decreased significantly than positive control (P<0.05), and Cinnamic aldehyde was better than Formocresol (P<0.05) (Fig.6, Fig.8). There were no significant difference of bacteriostat effect between 1mg/ml Paeonol and positive control (P> 0.05) (Fig.5, Fig.7).3 In the 7th day of root canal sealing agents, there was no fungal in root canal of Cinnamic aldehyde and Formocresol, bacteriostat effect was obvious (P<0.05) (Fig.10, Fig.12). lmg/ml paeonol group had obvious bacteriostat effect, the number of fungal in infected root canal decreased significantly than positive control (P<0.05) (Fig.9, Fig.11).Conclusion:1 Cinnamic aldehyde, formocresol and 1mg/ml paeonol have bacteriostat effect to candida albicans in root canal, Cinnamic aldehyde and formocresola are better than 1mg/ml paeonol.2 In the 7th day of root canal sealing agents, Cinnamic aldehyde and formocresol kill all the candida albicans in root canal and Superficial dentin.3. The bacteriostat effect of sealing medicament 7days is better than 3days. |
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