Experimental Study On Thymic Stromal Lymphopoietin In Mice Model With Atopic Dermatitis

Objective:To explore the role of thymic stromal lymphopoietin (TSLP) in the pathogenesis of atopic dermatitis (AD), the mice model of AD was established, the expression level of TSLP in the lesions from mice was tested, the expression levels of TSLP, interleukin 4 (IL-4), interferon-?(IFN-?) and immunoglobulin-E (IgE) in serum from mice were detected, the correlativity of expression level in serum from mice between TSLP and IL-4, IFN-y, IgE was analyzed. Methods: Eighteen female BALB/c mice were randomly divided into the experimental group (n=9) and the control group(n=9) according to randomized controlled experimental methods. Mice in experimental group were stimulated with 2,4-Dinitrofluorobenzene (DNFB) in acetone/olive oil (3:1). Meanwhile, mice in control group were only dealt with acetone/olive oil (3:1). The pathological changes of lesions in two groups from mice was observed with the method of hematoxylin-eosin (HE) staining. The expression of TSLP in the lesions from mice was tested by immunohistchemical technique. The contents of TSLP, IL-4, IFN-y and IgE in serum from mice were detected by enzyme-linked immunosorbent assay (ELISA). Data were analyzed by SPSS 17.0 software package.The t-test was used for the Comparison between the measurement data and Spearman correlation analysis was used for the associativity of each sample. Results:1.Skin lesions:Mice in experimental group appeared with Erythema, papules, scaling,crusting, Licheniflcation and so on;mice in control group showed no significant change.2. Organization changes in lesions:2.1 Patho-manifestation in lesions:When compared with mice in control group, Epidermis from mice in experimental group showed mild hyperkeratosis associated with parakeratosis, acanthosis, intercellular edema, and dermis layer from mice in experimental group showed inflammatorycells infiltration.2.2 Immunohistochemical manifestation:Positive particles were mainly concentrated in the Epidermis cells of the nucleus,the mean optical density of TSLP positive cells in experimental group was significantly higher than control group(p<0.05).3. The contents of TSLP,IL-4, IFN-y and IgE in serum:3.1 TSLP:The content of TSLP in experimental group(213.621±34.666pg/ml) was higher than that in the control group(151.997±22.328pg/ml), and there was significantly difference between the two groups (p<0.05).3.2 IL-4:The content of IL-4 in experimental group (97.831±15.882pg/ml) was higher than that in the control group(60.561±9.286pg/ml), and there was significantly difference between the two groups (p<0.05).3.3 IFN-y:The content of IFN-y in experimental group (70.999±9.064pg/ml) was higher than that in the control group (51.224±4.534pg/ml), and there was significantly difference between the two groups (p<0.05).3.4 IgE:The content of IgE in experimental group (142.488±16.352ng/ml) was higher than that in the control group(14.851±3.971ng/ml), and there was significantly difference between the two groups (p<0.05).4.Correlation analysis between TSLP and IL-4, IFN-y, IgE in serum.-There was positive correlation between TSLP and IL-4,IgE in experimental group (p<0.05).There was negative correlation between TSLP and IFN-? in experimental group (p<0.05).Conclusion:1.Mice Models of AD can be successfully established by DNFB sensitization.2.There is highly expression level of TSLP in lesions and serum from mice with AD.TSLP can promote IL-4,IgE expression and inhibit IFN-y expression.TSLP may play a key role in the pathogenesis of AD by inducing T help cell into Th2 cells.3. There are highly expression levels of IL-4,IFN-y and IgE in serum from mice with AD, it is showed these cytokines may also play an important role in the pathogenesis of AD.