| ObjectiveAtopic dermatitis（AD）,also known as atopic dermatitis and atopic eczema,is a common clinical inflammatory disease.Because of its easy recurrence and long duration,it seriously affects the daily life and psychological health of patients.The treatment of atopic dermatitis is longitudinal,but each has its own limitations and side effects,adverse reactions,etc.,and because the age and physical condition of patients are not the same,the medication is less targeted.The ancient Chinese healers referred to atopic dermatitis as Nai Xian.and considered it to be a combination of fetal toxicity and damp-heat evil.The chronic phase of atopic dermatitis is mostly caused by damp-heat injury and internal heat,with wind and dampness not yet resolved,so the method of nourishing blood,moistening dryness and removing dampness should be applied.It has been clinically observed that it can improve hypertrophic and dry skin lesions and reduce itching in AD patients.In this experiment,with the help of animal experiments,we aimed to investigate the mechanism of the mechanism of Fangji Dihuang Decoction in the treatment of atopic dermatitis.MethodsRepeated dorsal stimulation of BAlb/c mice with 2,4-2-nitrochlorobenzene（DNCB）was used to induce the formation of atopic dermatitis-like skin changes.A total of 50 6-week-old mice were randomly divided into the high-dose group of Fangji Dihuang Decoction,the medium-dose group of Fangji Dihuang Decoction,the positive control group,the blank control group and the model group after one week of adaptive feeding.From the second week of modeling,except for the model group and the blank control group,all mice were treated with the corresponding drugs by gavage.The mice were executed on the 28 th day and the skin and spleen were taken.During the experiment,the skin lesions of mice were observed and scored;the skin was stained for tissue sectioning to observe the skin condition;Rt-PCR was used to determine the differences in m RNA expression of relevant inflammatory factors in the skin lesions of mice,and the proportion of Th cells in the spleen of mice was analyzed by flow cytometry.ResultsAt the end of the modeling,compared with the mice in the blank group,the skin lesion scores of mice in other groups increased significantly,and the difference was statistically significant（P<0.05）.At the end of the experiment,the skin scores of the mice in the Fangji Dihuang Decoction group and the positive control group were significantly lower than those in the model group（P<0.05）.HE staining showed that the epidermis of the mice in the model group showed significant hyperplasia,hypertrophy of the spinous layer with spongiform edema,and a large number of inflammatory cells infiltrated in the epidermis and dermis.The histopathological spine layer hypertrophy was reduced in the mice given drug treatment compared with the model group,and the inflammatory cell infiltration in the skin was reduced.The rt-PCR assay of mouse skin tissues showed that the expression of IFN-and IL-4 was significantly upregulated in the model group,and significantly decreased in the high-dose group and the medium-dose group of Fangqi Dihuang Decoction and the positive control group（P<0.05）.ConclusionFangji Dihuang Decoction can effectively improve eczema-like lesions,reduce epidermal edema,exudation and pruritus in mice with atopic dermatitis model,and the effect of high dose of Fangqi Dihuang Decoction is similar to that of methylprednisolone.The mechanism may be related to the down-regulation of IFN-γ and IL-4 expression in the lesion tissue.The therapeutic effect of Fangqi Dihuang Tang can also be achieved by regulating the Th1/Th2 ratio and restoring immune homeostasis in vivo. |
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