The Enzymatic Extraction Of Epimedium Polysaccharide And Its Regulation On Animal Immune Function

Immunity is the main means for the defense of external pathogenic microorganisms by livestock and poultry,and it is also the basis for modern aquaculture care.Low immunity is one of the major causes of viral diseases and bacterial diseases in livestock and poultry,causing great losses to the livestock husbandry industry.Has a variety of physiological activities,in particular to promote the level of cellular immunity and humoral immunity,improve the body's immune system.Therefore,polysaccharides have excellent research value as an excellent immune adjuvant.Epimedium is a traditional Chinese herbal medicine,which has a wide range of pharmacological effects and clinical application value.Epimedium polysaccharide?EPS?is one of its main active ingredients,and its content is high.At present,there are few researches on the mechanism of immune promotion of Epimedium oligosaccharide at home and abroad,and it is necessary to further study its quality standard and its mechanism of promoting immune action in combination with modern pharmacology methods in order to make better use of it.In order to deeply study the immunological activity of Epimedium polysaccharides,the response surface method was used to investigate the enzymatic extraction process of Epimedium polysaccharose and the optimal extraction conditions were optimized.By orthogonal optimization,the transfer rate of polysaccharides was used as an index to explore the epimedium.The optimal alcohol precipitation conditions of Lycium barbarum.polysaccharides from the peritoneal macrophages to release NO and the proliferation of lymphocytes were taken as indexes to investigate the activity of epimedium polysaccharides obtained from different alcohol concentration and the effects of Epimedium polysaccharides on immune cells in vitro.Through animal experiments,we investigated the effects of Epimedium brevicornum polysaccharides on the antibody titer of Newcastle disease?ND?antibody and the structure of immune organs,systematically studied the mechanism of immune promotion of Epimedium brevicornum polysaccharides,and provided theoretical basis for the clinical access to new veterinary drugs for improving immunity.in accordance with.The test results are as follows:1?Response surface methodology optimization of cellulase-assisted extraction of epimedium polysaccharides:Cellulase-assisted extraction method was used to optimize the extraction factors through Box-Behnken's three-factor three-level response surface methodology,ANOVA analysis of variance results,and two-factor interactions.The 3D and contour plots showed that the optimum extraction conditions were cellulase concentration110 U/mL,enzyme extraction temperature 60°C,enzyme extraction time 100 min,extraction rate of Epimedium polysaccharide 10.06%,and prediction value 10.199.%is very close,reaching 98.6%of the forecast.2?Orthogonal design method to optimize the optimum alcohol precipitation conditions of epimedium polysaccharides:Analysis of variance by orthogonal test results on L₉?3⁴?showed that when the concentration ratio was 1:2,the ethanol concentration was80%,and the ethanol volume multiple was When the alcohol precipitation time was 12hours and the ethanol precipitation time was 12 hours,the metastatic rate of epimedium polysaccharide reached 72.27%,and the polysaccharide transfer rate was the highest.3?Epimedium polysaccharide stimulated macrophage release of NO in vitro test results:Epimedium Polysaccharides obtained from 5 different alcohol concentration can stimulate macrophages to release NO,the polysaccharide activity is best when the alcohol concentration is 50%,and with a dose-dependent effect,the release of NO increased with the increase of polysaccharide concentration.4?Determination of the maximum safe concentration of epilepsy polysaccharid-es on chicken spleen lymphocytes:The maximum safe concentration of epimedium polysaccharide was 2500.00?g/mL as measured by MTT and cytopathological observation.5?Effect of Epimedium Polysaccharide on Chicken Splenic Lymphocyte Proliferati-on Test Results:In the range of 312.50²500.00?g/mL,Epimedium Polysaccharide alone and synergistic LPS can significantly promote the proliferation of splenic lymphocytes with a concentration of 1250.00?g.At the time of/mL,spleen lymphocyte proliferation index and proliferation rate were the highest.6?Test results of epimedium polysaccharides promoting chicken immunity:?1?At different times after immunization,the antibody titer of Newcastle disease antibody was significantly higher in the high-dose group and the middle-dose group?P<0.05?than that of the blank control and the immune control group.?2?Histological observation of the immune organs of the thymus,spleen and bursa sac showed that epimedium polysaccharides can promote the development of immune organs.