Effect Of Astragalan Polysaccharide, Epimedium Polysaccharide And Epimedium Flavonaids On Activities Of NDV And IBDV

The crude Astragalan polysaccharide (APS) and Epirnedium Polysaccharide (EPS) were extracted with water and ethanol 1mm Astragalus root and Epimedium respectively. Epimedium flavone (EF) was extracted with water, ethanol, chloroform-acetic ester and by polyatuide filtration. APS and 1BPS were quantified by means of vitrol-anthracene ketone and EF, vitamip. The results showed that extractive rates of APS, EPS and EF were 2.6 gIKg, 5.2 g/Kg and 1.5 g/Kg, their net contents were 50.2%, 53.2% and 70.1% respectively. The highest yield rate of 1BPS was gained at 1:50 water dilution, 60% ethanol concentration and 4t sediment. The effects of APS and EPS on proliferation and survival time of CEF were observed and cytotoxicity of APS, 1BPS and EF for CEF were determined. The results indicated that APS and 1BPS could Stimulate the proliferation of CEF and made CEF survive longer time in which 1BPS was stronger and there was a dose-dependent relation. APS had not cytoxicity, while EPS and EF had cytoxicity at a higher dose which were 450 g / ml and 300 1 g / ml respectily. The effects of APS, EPS and EF on NOV and IBDV were studied by observation of cytopathic effect (CPE) and agglutination titer. The results showed that APS and EPS inhibited proliferation of NOV when they were added into the CEF ahead of NOV., and they did not when the addition later than NOV and after incubation. EF inhibited the proliferation of NOV and IBDV no matter how to add into the CEF. The effects of EPS, APS and EF on agglutination activity of NOV were determined. The results showed that experimental gioups were of higher agglutinated titer than control group(7times,3times and ltimes respectively). 1BPS could longthen agglutination time. EF presented a certain agglutinated activity at the higher concentration.