Sable(Martes zibellina)was a widespread mustelid,now only distributed in 6 countries.Because of habitat destruction and fragmentation,and the disturbance of human economic activities,the number of wild populations decreased sharply.The origin center of sable has been a hot issue.The south of Russian Far East and northeast of China has been repeatedly identified as origin center of sable.Present researches are limited to sable populations in its local range,lack of researches from global perspective.Or,simple genetic markers were used although sable population researched covered wild distribution areas.In view of this,complete mitochondrial genome sequences(without D-loop)of 44 sables and partial sequences of mitochondrial DNA control region of 43 sables were sequenced.All sables were sampled from Great Khingan Mountains,Lesser Khingan Mountains and Changbai Mountains of China,Mongolia,and Russian area adjacent to China.The genetic structure and evolution history of sable population was investigated by analysis of sequences obtained and 27 complete mitochondrial genome sequences of sables from Russia published on Gen Bank.The results are as follows:1.In this study,intraspecific differences mainly showed in ND2,ND3,ND5 gene and CYTB gene and control region.The AT-content was significantly greater than GCcontent,indicating an obvious AT preference.2.Genetic diversity is high in population of Great Khingan Mountains and Lesser Khingan Mountains,low in population of Changbai Mountains in China.In those area adjacent to China,genetic diversity is low in population of KMSU and KU,and high in population of UR,WS,BCK,PL and KR.3.Of the three branches involved in this study,population expansion was observed in branch A and B,but not in branch C.4.Based on the available data,it is suggested that the geographical populations of Greater and Lesser Khingan Mountains be considered as an independent ESUs,and population of Changbai Mountains as an independent ESUs. |