In Vitro Plant Micro Landscape Research Of Rosa Roelletti Products

In vitro plant micro landscape is a product concept independently proposed by our laboratory,it is a new ornamental product developed by plant in vitro culture technology.In vitro plant micro landscape is aimed at traditional plant micro landscape,which needs regular watering and fertilization,short life cycle and so on.In vitro plant micro landscape research of Rosa roelletti products is based on Rosa roelletti as the main landscaping material.By studying rapid propagation technology of five varieties of Rosa roelletti,the ornamental value of culture medium has been improved,and the design of micro landscape has been carried out.Finally,the Rosa roelletti products of detached plant landscape have been obtained.In vitro plant micro landscape research of Rosa roelletti products has provided technical support for Rose industry to increase the market shares.At the same time,it has promoted the development of Nanyang rose industry and provided new ideas for the development of other flower industries as well.In this study,the axillary buds of Rosa roelletti have been used as explants to sprout multiple buds,and by using the rapid propagation of regenerated those multiple buds,a large number of Rosa roelletti aseptic landscaping seedlings have been obtained.By studying the influence of the solidified agent on solidification and transparency of the culture medium,one type of transparent solid medium has been obtained.Through adding edible pigments to the medium,the ornamental value has been improved,and then screening those colors and different concentrations via questionnaires,the fixed formula of colored medium has been obtained.According to the growth curve of the aimed plants,the proper ornamental periods of those plants have been obtained.And designing the micro landscape,in vitro plant micro landscape products have been obtained.The main research contents and results are as follows:1.Study on tissue culture and rapid propagation of five Rosa roelletti CultivarsStems contained axillar buds from Rosa roelletti‘Nicole',‘Witte Koster',‘Dong Mei',‘Gold Sun'and‘Maidy',were used as explants for vitro rapid propagation.Steritization method of‘Nicole'and‘Witte Koster'was 75%ethanol?10 s?for surface sterilization,sterile water rinse 2 times;and then use the 0.1%HgCl₂ solution in the sterilization of 10min,washed by sterile water for 5 times,with a pollution rate of 17%.Sterilization method of‘Dong Mei'and‘Gold Sun'and‘Maidy'was that,first,75%ethanol was used for surface sterilization for 10 s,rinsed with sterile water for 2 times,using 5 min 10%NaClO solution sterilization,rinsed with sterile water for 3 times HgCl₂ solution,10 min 0.1%,rinsed with sterile water for 5 times,with a pollution rate of 8%.The optimutn medium for Rosa roelletti‘Nicole'and‘Witte Koster'and‘Dong Mei'and‘Gold Sun'and‘Maidy'adventitious bud induction was,MS+3%sucrose+0.8%agar,the optimum medium for proliferation was MS+1.5 mg/L 6-BA+0.3 mg/L IBA+3%sucrose+0.8%agar.2.Screening of transparent mediumThe degree of transparency of carrageenan,plant gel and agar,and the degree of coagulation of its added amount were compared.The gel of transparent medium was plant gel,the amount of which was 0.4%.The analysis of variance showed that the effect of transparent medium on the growth of plant material was not significant.So,plant gel can be used as a coagulant for gel medium in vitro micro landscape plants.3.Screening of color mediumChoose 7 kinds of edible pigments?Royal Blue,Paprika,Holly Green,Bitter Lemon,Velvet Red,White,Deep Purple?,according to the pigment concentration gradient change from shallow to deep as 8,18,30,66,102,180,300,420,660 mg/L,and the variance analysis showed that the color medium had no significant effect on the growth status of plant materials.The color medium can be used in the in vitro plant micro landscape.According to the questionnaires,this paper obtained in vitro plant micro landscape of the best viewing color and its concentration formula:MS+3%sucrose+0.4%plant+180mg/L Royal Blue,MS+3%sucrose+0.4%plant+300 mg/L Paprika,MS+3%sucrose+0.4%plant+660 mg/L Paprika,MS+3%sucrose+0.4%plant+180 mg/L Holly Green,MS+3%sucrose+0.4%plant+180 mg/L Bitter Lemon.4.The ornamental period and survival period of plant materialsAccording to the growth status and medium consumption of plant materials in tissue culture bottle,the best viewing period of camphor is after subculture for 20 d to 140 d,and the survival rate of a camphor sterile seedling in 50 mL medium could be about 540 d.The best viewing period of Rosa chinensis is after subculture of 10 d to 160 d,and the survival rate of a Rosa chinensis sterile seedling in 50 mL medium could be about 540 d.The best viewing period of tabacum is after subculture of 10 d to 160 d,and the survival rate of a tabacum sterile seedling in 50 mL medium could be about 360 d.The best viewing period of dendrobe is after subculture after 20 d,and the survival rate of a tabacum sterile seedling in50 mL medium could be about 720 d.According to the ornamental period and survival period of plant materials,it is easy to select plant materials for later plant micro landscape.5.Design and development of Rosa roelletti in vitro plant micro landscapeThis research used Rosa roelletti as landscape material,collocation transparent medium and color medium,three kinds of in vitro plant landscape Rosa roelletti theme products,“Mini Rose micro landscape”,“marine garden”and“small forest”were designed,with a unit price of 2.93 yuan and 8.16 yuan and 9.12 yuan respectively.