Study On The Extraction And Biological Function Of Uraria Crinita Flavonoids

In this study,after the extraction program of flavones was optimized,invitro antioxidation experiment,immune cell invitro cultivation and chemical liver injury test were carried out,exploring the biological function of the Uraria crinita flavonoids.1 optimization of the extraction process of the Uraria crinitaThe Uraria crinita roots were crushed,and then were pulverized by ultrasonic cell pulverizer(power 800W,10min,30s/times,each time interval of 15s).Univariate analysis experiment was conducted,considering the ethanol concentration,extraction time,extraction temperature and the solid-liquid ratio,to optimize the extraction process,and combined with orthogonal test,the optimal extraction condition was obtained:70%of the ethanol concentration,solid liquid ratio 1:25,extraction time 2h,extraction temperature 90?.In this condition,the extract ratio of the Uraria crinita flavonoids was 6353.18 mg/kg.2 the invitro antioxidant of the Uraria crinita flavonoidsThe Uraria crinita flavonoids was extracted,using petroleum ether,chloroform,ethyl acetate and n-butanol,to obtained the chloroform,ethyl acetate and n-butanol phase.Then the experiment of the deoxidization,lecithin liposomes antioxidant and superoxide anion radical(O2-)clearing were conducted.The conclusion was that in the concentration range used in the sample,there was a significant dose-dependent manner,when the concentration of the samples increase,the experiment data was also increase,and the effect of the ethyl acetate phase was relatively best.3 The effect of the Uraria crinita flavonoids on the mouse biochemical indicators84 mouse,weight 20g,were randomly divided into 6 groups:blank control group,model group,the positive control group that bifendate group(150 mg/kg),and Uraria crinita flavonoids high,medium and low dose group(450mg/kg,300mg/kg,150mg/kg).Carbon tetrachloride(CCl4)was used in the model group,and the mouse serum and liver tissue homogenates were used to the detection of the T-AOC?ALB?TP?AST?ALT?MDA?SOD and GSH-Px.The conclusion was that compared with the model group,the SOD,T-AOC,GSH-Px,ALB and TP level of the Uraria crinita flavonoids each dose group were significantly(P<0.05)or extreme significantly(P<0.01)higher,and the AST,ALT and MDA level were lower.Uraria crinita flavonoids can obviously effect on the repair of chemical liver.4 The effect of Uraria crinita flavonoids on the form of mouse liverThe liver of mouse were removed in the 4? saline to observe the form with eye,and then the suitable size of the organization was cut with a thin blade into the 10%formaldehyde solution fixed for 48h to produce the slice.The results showed that:compared with the model group,the liver form of the Uraria crinita flavonoids dose group observed with eye had no significantly pathological changes.In the microstructure,the pathological damage was minor,with varying degrees of recovery.The Uraria crinita flavonoids had a significantly protective effect on carbon tetrachloride induced liver injury.5 The effect of Uraria crinita flavonoids on immune parametersMTT assay invitro lymphocyte transformation test and the determination of peritoneal macrophage phagocytosis and acid phosphatase and lysozyme activity were conducted,the conclusion was that the Uraria crinita flavonoids dose groups could extremely significantly(P<0.01)improve the activity of the acid phosphatase and lysozyme,promote the T lymphocytes,B lymphocytes value-added,and also could significantly improve the phagocytic function of macrophages.