Production Of Lactic Acid Bacteria Fermented Solid Feed And Study On Its Effects On Growth Performance And Intestine Health In Piglets

Previous studies showed that lactic acid bacteria can produce large amounts of lactic acid and other organic acids,which may decrease the feed pH,as well as increase the number of probiotics and decrease harmful bacteria in feed.In this study,Lactobacillus salivarius L2,a lactic acid bacteria strains,was inoculated in feed to make the fermented solid feed,and its nutrient,storage methods,and its effects on growth performance and small intestine health in piglets were also studied.1 Characterizing the nutrient of fermented feed inoculated by lactic acid bacteriaIn this study,lactobacillus salivarius isolates L2 were selected as the inoculum for fermentation feed production,and the complete feed without antibiotics was used as substrate.The conditions of fermentation including temperature and time was characterized,and the ratio of nutrition,dry matter,pH,the concentrations of lactic acid and volatile fatty acid,and the counts of lactic acid bacteria and E.coli in the fermented feed were then compared between the control and treatment.The results show that the optimal temperature and incubation time is 37℃ and 48 h for producing the fermented feed.As compared with the control,the nutritional composition,the content of crude fiber and the pH in fermented feed is significantly lower(P<0.05),while the protein and fat level is similar(P>0.05),the concentration of lactic acid in fermented feed was 141.53 μmol/g,which was significantly higher than control group(23.65 μpmol/g)(P<0.05).There was no difference(P>0.05)in the VFA concentration(except for the propionate and isovalerate)between this two groups.The counts of lactic acid bacteria was increased(P<0.05),while E.coli was decreased(P<0.05)when compared with control group.These results indicates that the L2 strain inoculated in the feed can produce a higher concentrations of lactic acid and the counts of lactic acid bacteria,inhibiting the counts of E.coli.These results suggest L2 has the potential for use as inoculant in fermented feed.2 Study on the effects of different antiseptic in preventing the growth of mold in the fermented feedIn this study,four types of antiseptic,including calcium propionate,citric acid,sodium diacetate(SDA)and potassium sorbate,were added in the fermented feed to measure its antiseptic effect.The fermented feed containing antispetic were kept in Bio-bag and culture dish,respectively,and were incubated in a condition of 28 °C and 72%relative humidity for 30 days.The results show that,under the condition of unsealed(culture dish),the antiseptic effect of SDA and calcium propionate were better than other two group,while under the condition of sealed,all of these four antiseptics show a better ability on antiseptic.In the minimal inhibitory concentration test,2.00%calcium propionate or 0.30%SDA into fermented feed could fully prevent the growth of mold within 6 days.In general,these results suggest that 2.00%calcium propionate or 0.30%SDA is a better antiseptic in fermented feed.Based on the dosage and economy,these results showed that SDA can be used to inhibit the growth of mold in storing the fermented feed.3 Effects of fermented feed on growth performance and intestine health in weaned pigletsThe objective of this study was to investigate the effects of fermented feed which was mentioned in experiment on rowth performance and intestine health in weaned piglets.24 weaned piglets[Landrace×(Yorkshire×Duroc)]weaned pigs were randomly allotted to two treatments,on the basis of body weight and sex in a randomized complete block design.There were 4 replicate pens per treatment and each pen comprised 6 pigs.Pigs in control group were fed with basal diets and treatment group were fed with 80%basal diets and 20%fermented feed.This experiment last for 29 days,and the hole cycle was divided into two period,including the firestl-14 day period and second 15-29 day period The results showed:(1)In the first period there is no significant difference on average daily feed intake(ADFI)average daily gain(ADG)between two group(P>0.05),and in second period,piglets fed with fermented feed had higher ADFI and ADG compared with control group(P<0.05),in the first period,fermented feed had no significant influence on ADG and feed intake/weight gain(P>0.05),however,higher ADFI was observed in test group(P<0.05);(2)There is no difference on the weight of immune organs between two groups(P>0.05);(3)NO significant difference was observed on pH value of archenteric chyme(P>0.05);(4)There is no difference(P>0.05)in the VFA concentration[except for the acetic acid and propionic acid(P<0.05)]in the colonic digesta,while the VFA concentration in the jejunum has no difference;(5)Pigs fed with fermented feed had no higher villous height and crypt depth(P>0.05),neither the ratio of them(P>0.05);(6)Pigs fed with fermented feed had lower counts of E.coli.(P<0.05),higher counts of lactic acid bacteria(P<0.05),while had no significant influence on the counts of salmonella.These results suggest that the fermented feed incubated by lactic acid bacteria could improve the gut health of gastrointestinal tract,while show no significant effect on growth performance in piglets.