Effects Of Baicalein On IL-1?-induced NF-?B Pathway In Articular Chondrocytes

Osteoarthritis(Osteoarthritis,referred to as OA)is a chronic joint disease,which clinical symptoms reminds joint degeneration of bone.When the animals occur OA,they will have a series of dysfunction,which appears pain and swelling of bone and joint,which makes the labor value of the animals and brings economic losses.At present,the clinical treatment of OA is mainly from the following fields,such like: the reduction of the pain;ease of the symptoms.They offen make the affected animals taking acetaminophen,non-steroidal anti-inflammatory drugs(NSAIDs),selective cyclooxygenase(COX-2)inhibitors and other drugs.Or the affected animals will be injected with glucocorticoids,hyaluronic acid and other methods in intra-articular.These methods do can alleviate the clinical symptoms of OA,but with varying degrees of side effects.OA's treatment should not only improve the symptoms,but also to achieve the purpose of eliminating and preventing OA.Therefore,the clinical study of treatment of OA,will be further elucidated its pathogenesis.In addition,the need to develop a safe and effective drugs with no toxic side effects of OA seems so importent.In recent years,plant extracts becomes as a hot topic,because of its indeed efficacy and its small side effects in OA's treatment.Baicalein(BAI)is a flavonoid compound extracted from the roots of Scutellaria.It has been proved that it has a variety of biological activities,such as anti-inflammatory,anti-fibrosis and anti-apoptosis.At present,many scholars believe that the pathogenesis of OA is due to cytokines or inflammatory mediators and other factors co-role in chondrocytes,resulting in chondrocyte apoptosis and cartilage matrix degradation caused.The proinflammatory cytokine IL-1? produced by chondrocytes during the pathogenesis is confirmed by involvement in chondrocyte apoptosis and activation of the NF-?B signaling pathway.Therefore,in this study,IL-1? was used to stimulate rat chondrocytes and induced to produce OA model.BAI extract was applied to cartilage cells of this model to explore the effect of BAI intervention on IL-1?-induced rat OA-?B signaling pathway and its gene product expression,and to elucidate its mechanism for the future clinical BAI as a drug for the treatment and prevention of OA provide a theoretical basisIn this study,chondrocytes from lactating young rats were pretreated with 10 ng / ml IL-1? for24 h and then treated with 10 ng / ml IL-1? and 50 ?M baicalein 0,4,8,12 and 24 hours.The effect of baicalein on the viability of chondrocytes was detected by CCK-8 kit.Western blot was used to detect the expression of matrix metalloproteinase-3,MMP-9 and COX-2 in chondrocytes.The effect of BAI on the expression of NF-?B p65 and phospho-NF-?B p65 in cytoplasm and nucleuswas detected by Western Blot.The effect of baicalein on nuclear translocation of NF-?B was detected by immunofluorescence.The results showed that compared with the control group,different concentrations of(5,10,25,50,100 ?M)baicalein were not incubated with chondrocytes for 12,24 and 48 h,respectively.Western Blot test showed that BAI could inhibit the expression of MMP-3,MMP-9 and COX-2,inhibit the phosphorylation and nuclear translocation of p65 in cytoplasm.This suggests that BAI can inhibit the apoptosis of chondrocytes and the degradation of cartilage matrix in IL-1?-induced rat joints.The protective effect of BAI can inhibit the activation of NF-?B signaling pathway by inhibiting the nuclear translocation of phosphorylated p65.