The Varieties Of Silk Lan Ni Kang Rapid Propagation Technology Research

Hydrangea L.is a woody plant with an important ornamental value in the Saxifragaceae,with totall 85 kinds of varieties distributing in the east to southeast of Asia,southeast of North America to the central America and west of south America.While in our country there are 46kinds of varieties with 10 variations which is distributed all around China except the southern hainan,northeast of heilongjiang,jilin,northwestern of xinjiang and other provinces.It is mainly produced in the south of Qinling Mountains,especially from its southwest to the southeast with many kinds of varieties.The paper analyze on the results of experimental on three levels as cuttage and tissue culture a good variety of'Hydrangea macrophyllaon' which is imported abroad in order to perfect the propagation technique system of 'Hydrangea macrophyllaon'.Results as below:1.The experiment on cuttage shows:it is the NAA acid that has a big impact on the root length,root number,and even the survival rate of 'Hydrangea macrophyllaon'.It is the pearlite that is fit for its cuttage matrix:with sand 1:1,survival rate as 85%,average root number is 7.6,and the root length is 2.6 cm.It is the NAA that is the best suitable to its cuttage among three kinds of plant growth regulator of 500mg/L,with survival rate 93%,the average root number 7.9,average root length 2.1 cm;while the NAA concentration is 600mg/L,survival rate is 93%,the average root number is 8.3,average root length is 2.3 cm.2.The best method of disinfection for the leaves of 'Hydrangea macrophyllaon' is to use the water to swatch it for 30-60 min,then put it into the 75%ethyl alcohol for sterilization,and flush the explant 4 to 5 times using sterile water.Then use mercury with the concentration of 0.1%to sterilize for 14min on the super clean bench.At last,flush its leaves 4 to 5 times using sterile water,dry it by filter paper,and inoculate to the original medium for culture.3.In the experiment on the tissue culture of 'Hydrangea macrophyllaon',the best culture medium component by callus induction is MS+IBA1.0 mg/L+NAA1.0 mg/L,there is a growing callus after 12d,with the callus induction rate up to 89.16%;The best rooting medium is MS+IBA1.0 mg/L,with rooting rate 100%,average root time 6.7d,root length 2.0cm;while the best optimum culture medium of axillary bud induction for it is MS+6-BA 1.0 mg/L,with the inductivity of adventitious bud 92.5%,and each of the average number of explant adventitious bud 3.3 pieces.The seed germination medium on the embryo rescue breeding technology is MS+NAA 1.5 mg/L,seeds begin to sprout after 11d,with the germination rate up to 82.5%.