Characteristics Of Organ Indices, Intestinal Morphology And Gastrointestinal Contents Ph In Diarrhea Piglets And The Effect Of Drenching With Of Enterococcus Faecalis On Them

Currently,diarrhea is a major reason for the death in piglets,which has an importantimpacnt on the development ofgastrointestinal tract.As a substitute of antibiotic,probiotics have been used to prevent and control diarrhea.However,the efficiency and dose of probiotics still need to be further studied.In this study,heart,liver,spleen and kindey index,small intestine morphology and pH of gastrointestinal contents in diarrheal piglets as well as effects of drenching with Enterococcus faecalis on piglets,were analyzed to reveal effects of probiotics on piglets especially diarrheal piglets,offer theoretical basis for the application and spread of probiotics in the future.The study was conducted among 48 litters of piglets.Physical condition of piglets was carefully observed in the first stage(3-7days postnatal).Once a piglet was found diarrhea,it was weighed,slaughtered and sampled at once.Another nondiarrheal piglet from the same litter with the same gender was also weighed,slaughtered and sampled until samples from 20 litters were collected.In the second stage(8-20days postnatal),all of the piglets left in those 20 littlers were fed till 20days postnatal i.e.the day before weaning.Piglets with similar date of diarrhea,birthday,litter size were allocated into five groups with four replicates each.Piglets in control group(C)were drenched with 1mL double-distilled water at the 1st day,3rd day,6th day of the diarrhea,respectively.Meanwhile,piglets in the other four corresponding treatment groups(T1-T4)were drenched with 1mL probiotics at the levels of 2.5 x 108 CFU/mL,5 x 108CFU/mL,1×109CFU/mL,2×109 CFU/mL at the same time,respectively.The results showed that:1.There were no significant differences between diarrheal and non-diarrheal piglets in the weight of liver,spleen,kidney and organ index,as well as pH value of stomach,small intestine and colon,other parts of large intestine.But there was significant difference in the pH value of colon.Villus height of duodenum and the ratio of villus height and crypt depth in diarrheal piglets were both significantly shorter than that in non-diarrheal piglets(P<0.05).There was no difference in morphology of the other parts of intestine.2.There were significant differences between diarrheal and non-diarrheal piglets in heart and kidney index drenched with Enterococcus faecalis probiotics(P<0.01).The spleen index in T1,T3,T4 were higher than that in C group,and lung index in T4 was higher than that in C group(P<0.05).Drenching Enterococcus faecalis probiotics decreased liver index of piglets,and the value of T1 was lower than that in C group.Diarrhea and interaction of diarrhea and treatment had no effect on heart,liver,spleen,kidney and lung index(P>0.05).Treatment had significant effect on pH value of ileum,the value of T3,T4 and Tlwere higher than that of C group(P<0.05),and the value of T3,T4 were higher than that of T1.Villus height in jejunum of T3 and T4 were higher than that in C group(P<0.05).But treatment,diarrhea and interaction of treatment and diarrhea had no effect on villus height,crypt depth and the ratio of villus height and crypt depth(P>0.05).