Effects Of Fructus Hordei Germinates And Glutamine On Growth Performance, Antioxidant And Immunity Of Weaning Piglets

The objective of this study was to compare the difference of fructus hordei germinates and glutamine on growth performance, antioxidant and immunity, to study the feasibility of the substitution of glutamine for fructus hordei germinates. Fourty-eight DurocxLandracexYorkshire weaned piglets which weight 8.23±1.06 kg were selected and randomly divided for four groups(each group of 3 repeat, each repeat have 4 pigs) according littre and weight. The four groups were treated 15 day trial with control group (basal diet), fructus hordei germinates group (basal diet+1% fructus hordei germinates), glutamine group (basal diet+1% glutamine) and composite group (basal diet+1% fructus hordei germinates+1% glutamine). On the 8th day and 15th day of the experiment, weighting the fasting piglet and feed consumption of per repeat, calculating its production; randomly selecting two piglets of each repeat with anterior vena cava blood and detect alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), blood urea nitrogen (Urea), total cholesterol (TCH) and 1gG and conventional indicators of white blood cell count (WBC), red cell count (RBC), hemoglobin (HGB), hematocrit (HCT), platelet (PLT); collecting faeces in each pens on 14th and 15th days and determined apparent digestibility of total protein (TP), total fat (EE), calcium (Ca) and phosphorus (P) by the method of endogenous indicator (AIA); Determination of multiple enzyme activities in liver based on various Kit; Determination of mRNA expression of gastrin and interleukin-6 on stomach and duodenum on piglets by the real time fluorescence quantitative method. The result showed that, (1)With the grow time of weaning piglets, daily gain and daily feed intake increased, feed conversion ratio decreased. In the first week and second week after weaning, the glutamine group was higher than other groups on daily gain and daily feed intake (the daily feed intake on 1 d-7 d of control group exception); the fructus hordei germinates group is the largest decline with time for feed conversion ratio; the composite group could effective reduce the number of diarrhea over time; Glutamine group and composite group can significantly (P<0.05) reduce the apparent digestibility of Ca; the fructus hordei germinates group can significantly (P<0.05) increased the apparent digestibility of P. The expression of gastrin mRNA in stomach and duodenal of the composite group and glutamine group was significantly higher than the fructus hordei germinates group (P<0.05) at the 7th day and 14th day. The expression of gastrin mRNA in duodenum of composite group was very significantly higher than the other groups; Differences in each group of blood (HGB?HCT?PLT) is not significant(P>0.05), the composite group in HGB?HCT?PLT is higher than single group, the fructus hordei germinates group of ALT and TCH can significantly (P<0.05) higher than the composite group and glutamine group. (2) The content of T-AOC and GSH-Px in glutamine group is significantly (P<0.05) lower than other groups, and than the rise is higher than other group; the content of CuZn-SOD and MDA in glutamine group is separation higher and less than other groups. (3) The effects of WBC, RBC and IgG on weaning piglets in each treatment group were not significant. There is no significant difference on each adding groups of IL-6 mRNA expression after weaning 7 days, the glutamine group and composite group was significantly (P< 0.05) higher than the fructus hordei germinates group and control group of IL-6 mRNA expression on stomach after weaning 14 days. Conclusion:The effect of glutamine on the performance and immunity of weaned piglets was the best, and the antioxidant properties of the composite group was the best.