| HuPing mountain, which has various archaic and precious plants, is located in Changde, Hunan province. Tea is a good local development of industry. Camellia sinensis belong to Camellia, which is a perennial cross-pollination evergreen. It has two different progenitive modes, including sexual propagation and asexual reproduction. Sexual reproduction is also called seed reproduction and leads to genetic diversity and can be applied for germplasm innovation and research. Breeding of fine tea cultivars with high yield and good quality, multi resistance, is the goal of tea breeding experts.Substance of molecular markers is that genetic polymorphism can be expressed in DN A level because of specific difference DNA fragment between biont and population. DNA molecular marker is more superior to the morphological marker, cytological marker and biochemical marker. Microsatellite-anchored fragment length polymorphism (MFLP) technology is combined with markers and molecular markers SSR dual principle. It, belonged to wet codominant markers, does not need the prophet genome information, has rich polymorphism, and is apt to transform specific nsequence reliably and repeatable, In this study, a fluorescent microsatellite-anchored fragment length polymorphism (MFLP) technique was established by adding universal M13-F-IRDye700 (5’-CACGACGTTGTAAACGAC-3’) adapter to the anchored primers. Six highly polymorphic primer pairs were selected from 324 selective amplification primer combinations, and were used to screen polymorphisms for 44 genotypes of flowering Tea. The genetic similarities were calculated and showed their distribution ranged from 0.1 to 0.58 among the 44 genotypes,288 Bands were produced by the 6 primer paris, of which 232 bands were polymorphie, the percentage of polymorphism was 80.6%. The genetic similarities were calculated and showed their distribution ranged from 0.1 to 0.58 among the 44 genotypes. This suggests that there are differences in the genomes of different sexual reproduction groups in the same geographical position. |
