| The objective of this study is to compare the difference of Malt and Gln on intestinal digestion and barrier function of weaned piglets. A total of 48 piglets, weaned at 21 d, were randomly allocated to 4 dietary treatments:control (basal diet without Malt or Gln), malt(basal diet+1%malt), Gln(basal diet+l%Gln) and mixed (basal diet+1%malt+l%Gln)treatment. Each treatment had 3 replicated pens with 4 pigs per pen. Individually pened pigs were slaughtered on 7 d and 14 d of test. The activity of intestinal digestive enzyme, intestinal morphology, expression of IgA protein and cytokines(IL-2, IFN-γ and IL-1β) in intestinal tissue of weaned piglets were analyzed by enzymology, histology, electron microscopy, immunohistochemical staining and ELISA. The statistical findings were as follows:1) Both of malt and Gln could improve the activity of the intestinal digestive enzyme. Compared malt treatment with Gln treatment,malt could significantly increase the activity of the duodenal trypsin,duodenal and jejunal lipase on the 7th day of experiment (P<0.05);malt significantly increased the activity of the duodenal trypsin and lipase,the jejunal trypsin on the 14th day of experiment (P<0.05);Gln significantly increased the activity of the intestinal amylase(P<0.05).2) Compared with the control treatment, malt treatment and Gln treatment could significantly improve the intestinal VH and V/C(P<0.05). During the whole experiment period, duodenum VH in Gln treatment significantly higher than that in malt treatment (P<0.05), ileum VH in malt treatment significantly higher than that in Gln treatment(P<0.05), jejunum VH and intestine V/C in malt treatment and Gln treatment showed no significant difference(P>0.05).3) Compared with the control treatment, malt treatment and Gln treatment increased the content of IgA protein and cytokines in intestinal tissue. Compared malt treatment with Gln treatment, Gln significantly increased the expression of duodenal IgA protein on the 7th day of experiment(P<0.05),while they showed no significant difference on the 14th day of experiment(P>0.05); Gln increased content of IL-2 and IL-1β in duodenum during the whole experience period, whereas malt treatment increased the content of IL-2, IFN-y and IL-1β in jejunum and ileum on the 7th day as well as ileum on the 14th day of experiment.4) Compared malt treatment, Gln treatment with mixed treatment, there was no significant difference in intestinal V/C, the expression of jejunal IgA protein, the activity of the jejunal trypsin and amylase between the mixed treatment and the other two treatments on the 7th day of experiment(P>0.05); similarly, there were no significant difference in VH, V/C of jejunum and ileum, the expression of intestinal IgA protein, the activity of the jejunal trypsin and lipase between the mixed treatment and the other two treatments on the 14th day of experiment(P>0.05); during the whole experiment period, the content of IL-2 in jejunum and ileum, IFN-y of duodenum and jejunum, IL-1(3 of duodenum and ileum in mixed treatment showed no significant difference with the other two treatments(P>0.05).Conclusion:In the matter of the intestinal digestion,malt was better than Gln.Both of malt and Gln could improve the intestinal morphology and promote intestinal immune response of weaned piglets. Their sites of action on the intestinal are different, Gln acts on the duodenum, while malt acts on the jejunum and ileum. |
