Virus Elimination And Rapid Propagation Of Small Yellow Ginger (Zingiber Officinale Rosc) In Guizhou

Ginger is a very important cash crop with higher values in food and medicine.Due to the long-term asexual reproduction, ginger accumulates a variety of virus and bacteria. The yield and quality of ginger are much higher for the virus free plants and bacteria free plants than that of the normal one in ginger production.The technology of virus-free and bacteria-free ginger seedling and its rapid propagation via tissue culture were studied deeply in this thesis,and genetic stability evaluation to the in vitro plantlets was carried out in this present study, the promotion systems of virus-free technology and ginger seminal seedlings were discussed in order to resolve the problems during ginger production via improve ginger rhizome quality and quantity.The main results are as follows:1. When culture temperature was 28? and culture substrate was perlite, the number of sprout in female ginger was the highest. proper sterilization time was good for the survival rate of stem tip,the disinfection treatment with0.1%Hg Cl2 for 15 min and 75% alcohol for 1min were the best, contamination rate of ginger was 17.1%.2. Studies on rapid propagation technology of ginger seedlings: the virus and bacteria of ginger(Guizhou-ginger) was eliminated by stem apex cultured in vitro.The highest regeneration frequency 73% was observed when explants cultivated on the inducing medium of MS supplemented with 3.5mg/L6-BA and 0.1mg/L NAA for shoot organogenesis. MS supplemented with 3.0mg/L6-BA and 0.2mg/L NAA was the best subculture and proliferation medium.3. Virus of ginger was determined: Virus(tobacco mosaic virus, TMV and cucumber mosaic virus, CMV) was determined by ELISA(Enzyme-linked immunosorbent assay) among the ginger seedlings derived from tissue culture, and the seminal ginger seedlings free of virus were obtained at last.4. Studies on hardening and transplanting technology: Open the bottle,ginger seedlings hardened eight days were transplanted to different substrates at room temperature,the tissue culture was transplanted to nutrient soil+perlite 3:1 mixing matrix,the survival rate reached 94% and its leaves grew very well.5. Genetic stability assessment of in vitro shoots: Using 23 primers, ISSR markers were employed to evaluate the genetic stability of different cycles of sub-cultured and different lines. No DNA variation was scored in the in vitro plants that had been transferred many as 5 as cycles.