Assessment Of Genetic Stability In Tissue-cultured Products And Seedlings Of Saussurea Involucrata By RAPD And ISSR Markers

To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant,Saussurea involucrate Kar.et Kir.,DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA(RAPD) and inter-simple sequence repeats (ISSR) markers.The seed plantlet of S.involucrata were used as materials of extracting DNA.The percentages of polymorphic bands in the RAPD and ISSR analysis were,respectively,35%and 33%. Cluster analysis indicated that the genetic similarity values calculated on the basis of RAPD and ISSR data among the 11 type of plant materials were respectively ranged from 0.823 to 0.995 with a mean of 0.878 and 0.825 to 0.974 with a mean of 0.885,which classified the samples into three groups.So, apparent genetic variation was detected in the 11 type of plant materials.And results provided a reference for tissue culture,artificial cultivation and sustaining usage of resource of S.involucrata.