| In this study,Chinese medicine extracts of Radix Codonopsis,Rhizoma Atractylodis Macrocephalae,Poria,Radix Glycyrrhizae,Fructus Crataegi Pinnatifidae,Massa Medicata Fermentata,Radix Angelicae Sinensis,Radix Astragali Mongolici and so on,were processed into a functional additive and added to canine food,to investigate the therapeutic effect of the Chinese medicine prescription on dogs with spleen deficiency,and evaluate its application security.Experiment ? : 24 local hybrid dogs with the similar weight were randomly divided into control group,natural recovery group,high-dose Chinese medicine group and low-dose Chinese medicine group,6 dogs per group.Dogs in natural recovery group,high-dose and low-dose Chinese medicine groups were hypodermically injected with reserpine to develop spleen deficiency model.After the model developed,dogs in high-dose and low-dose Chinese medicine groups were fed daily with the diets which contained Chinese medicine extracts of 3 and 1.5 g·kg BW-1raw Chinese medicine for 7 d,respectively.Blood samples were taken from forelimb vein of dogs at d 7 before and after modeling and d 7 after treatment,and feces were collected at d 4-6 after modeling and d 4-6 after treatment,to measure the blood physiological and biochemical index,as well as crude protein,dry matter and ash apparent digestibility.Experiment ? : 24 healthy local hybrid dogs with similar weight were randomly divided into control group and Chinese medicine group,12 dogs each group.Dogs in Chinese medicine group were fed daily with the diet which contained Chinese medicine extract of 1.5 g·kg BW-1 raw Chinese medicine.The trial was conducted for 28 d.Blood samples were taken from forelimb vein of dogs at d 0,7,14 and 28,and feces were collected at d 4-6,11-13,18-20,and 25-27,to determine the blood physiological and biochemical index,as well as crude protein,dry matter and ash apparent digestibility.Experiment ? : 48 healthy Kunming mice were randomly divided into four groups,12 mice each group,half male and female,were used to carry out the acute toxicity test according to traditional Chinese medicine toxicology test methods for 7 d.24 healthy Kunming mice were randomly divided into two groups,12 mice per group,half male and female,were used to carry out the maximum tolerated dose trial according to traditional Chinese medicine toxicology test methods for 7 d.48 healthy Kunming mice were randomly divided into four groups of 12 mice each,half male and female,were used to carry out the subchronic toxicity test according to traditional Chinese medicine toxicology test methods for 30 d.The results were as follows:1.Compared with the control group,canine blood lymphocytes,red blood cell,mean corpuscular volume,hemoglobin,glucose,Ig G,Ig A,Ig M,lysozyme,motilin,gastrin,serum amylase activity,serum D-xylose content and crude protein,dry matter and apparent digestibility of ash were significantly decreased 7 d after modeling(P<0.05);blood neutrophil,mean corpuscular hemoglobin concentration,and serum triglyceride,total protein,albumin,aspartate aminotransferase,total bilirubin,urea nitrogen contents were significantly increased(P <0.05).Compared with the control group,7 d after treated with Chinese medicine,canine erythrocyte,total bilirubin,amylase and Ig M levels in low-dose group had significant difference(P <0.05);and the above indexes in high-dose group had no significant difference(P >0.05);while part of the indexes in natural recovery group had significant difference(P <0.05).2.Compared with the control group,canine blood routine examination,liver and kidney functions showed no significant difference in Chinese medicine groups(P>0.05);serum D-xylose,Ig G and Ig A levels were significantly increased(P <0.05),and apparent digestibility of crude protein,dry matter and ash were significantly enhanced(P <0.05).3.The liver and kidney function indexes of mice in each group showed no significant difference(P >0.05)according to acute toxicity test,the maximum tolerated dose test and subchronic toxicity test.Meanwhile,liver and kidney cells were normal by histopathology.In conclusion,these experimental results confirm that the Chinese medicine preparation can effectively improve canine spleen deficiency symptoms,enhance digestive and absorptive function and immunity.The Chinese medicine preparation is safe and non-toxic in dogs. |
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