| This study was conducted with fusarium toxin and modified adsorbent(Calibrin-A, CA) as the research object. We used the solid-state fermentation method to analyse the change rules of fermentation feed products, and to summary the effect of fusarium toxins and modified adsorbent on the fermentation products. We expected that the results could provide theoretical basis for scientific production of fermented feed.The experiment was divided into 6 treatments: The control group was the basal diet. The test group 1(25Myco.) and 2(50Myco.) were the basal diet that corn and corn gluten meal were replaced by 25% or 50% naturally moldy corn and corn gluten meal, respectively. The test group 3, 4 and 5 were the basal diet, test group 1 and 2 with the addition of 0.2% CA, respectively. Compound strains(lactic acid bacteria, 6.33×106 cfu/g; yeast, 1.05×106 cfu/g; bacillus subtilis 8.94×106 cfu/g) were used by solid-state fermentation of the compound feed. Before the start of fermentation, water of all processed diet was adjusted to 50%. Results showed that dietary p H, DM, starch, reducing sugar and ZEN content decreased significantly(P < 0.05) along with the fermentation time; but CP, total P, lactic acid, NH3-N, DON and FB1 increased significantly(P < 0.05). Acetic acid and butyric acid were increased first and then decreased(P < 0.05). The p H, NH3-N, lactic acid and acetic acid of diet(0 d) decreased significantly with the elevated levels of fusarium toxins(P < 0.001), but the reducing sugar and total P of the control were significantly lower than both two toxin groups(P < 0.05). At the end of fermentation(18 d), the p H and NH3-N of the control were significantly lower than both two toxin groups(P < 0.001). The reducing sugar of the 50% toxin group was significantly higher than both control and 25% toxin groups(P < 0.05). The starch in the control group was significantly higher than that in the 50% toxin group(P < 0.05). The lactic acid of diet decreased significantly with the elevated levels of fusarium toxins(P < 0.001). The average p H, lactic acid and acetic acid of the whole fermentation process decreased significantly with the increasing of toxin levels(P < 0.001); The average NH3-N and butyric acid of the whole fermentation process in the control was significantly lower than both two toxin groups(P < 0.001), but the average starch in the control was significantly higher than both two toxin groups(P < 0.05). The average total P of the whole fermentation process in the control was significantly higher than 50% toxin group(P < 0.05). The average reducing sugar of the whole fermentation process in the 50% toxin group was significantly lower than both control and 25% groups(P < 0.05), but the Ca was in contrast(P < 0.001). The average butyric acid, ZEN of the whole fermentation process of CA treatment was significantly lower than that without CA treatment(P < 0.05). The significant interaction of fusarium toxin and CA on the p H(6 d, 9 d), lactic acid(12 d), acetic acid(9 d), butyric acid(12 d, 18 d), DON(18 d) and the average DM, reducing sugar, acetic acid and butyric acid of the whole fermentation process were observed(P < 0.05). Pearson analysis showed that p H was significantly positively correlated with DM, reducing sugar and starch, and p H was significantiy negatively correlated with lactic acid and acetic acid. DM was significantiy negatively correlated with CP and NH3-N.In conclusion, under the experimental conditions, the fermentation products of p H, NH3-N, reducing sugar, starch, lactic acid, acetic acid, butyric acid, Ca and total P of the compound feed were significantly affected by fusarium toxin and its levels. Adding CA significantly affected the fermentation products of acetic acid and butyric acid, and fusarium toxin and CA had significant interaction on p H, DM, reducing sugar, lactic acid, acetic acid, butyric acid and DON. Mycotoxin of ZEN was significantly reduced after fermentation, while DON and FB1 content increased significantly. There are different degrees of correlation between the products of fermentation. |
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