| Pepper(Capsicum annuum L.) has become one of widely used and cultivation vegetables in the world because of its unique taste and nutrition, with the continuous improvement of people's living standard, the requirement of vegetables is also higher, they not only have more and more requirements for nutritions of vegetables, but also pay more and more attention to the appearance of it, the breeding of purple vegetables catered to people's dual requirements of appearance and nutrition. Purple peppers have rich anthocyanins which have very large nutrition health care function. The expression of anthocyanin biosynthesis genes and inheritance of purple properties in purple pepper was analyzed, main results are as follows:1. In order to clarify the expression and regulation patterns of related gene in the process of anthocyanin biosynthesis in pepper, select three pepper strain which fruit of different color for materials ('Zijing','Danzi','Shuijing'), extract RNA of different stages of the fruit and reverse transcribed into cDNA, then using qRT-PCR technology to analysis of expression of structure gene (CHS?CHI?F3H?F3'5'H?DFR?ANS?UFGT?ANP and GST) in pepper fruit of three kind materials in different development stages. the results showed that:the expression of CHS, CHI are rarely and disorder in the process of fruit development of three kind materials, the expression of F3H is higher in 'Danzi' and 'Zijing', and appear larger peak in the fruit development of 15-20 days, compared with it, the amount of expression in 'Shuijing' can be negligible. The relative transcription level of F3'5'H?ANP?GST?DFR and UFGT express synchronous change in three kinds of materials, and the relative expression of F3'5'H, ANP and GST in the 15th day of 'Zijing' fruit appeared larger peak, the relative transcription level of ANS without some rules to follow. F3H, F3'5'H?DFR?UFGT, ANP and GST are the key genes to the anthocyanins metabolic pathway in pepper.2. With purple pepper which have fuzz 'Zirong' and green pepper without fuzz 'Shuijing'as parents, hybrid get F1, F1 selfing get F2, statistics main characters of F1 and F2 (stem color, leaf color, leaf vein color, stem fuzz, petal color, semet color, stigma color, style color, flower stalk color, fruit color, fruit shape, fruit growing direction), to analysis genetic law of purple traits in pepper. Results showed that:with 'Zirong' as female parent, filial generation F1 expression is purple,'Shuijing' as the female parent, filial generation F1 basic all green. With 'Zirong' as the female parent descendant F2 populations, plant trait which same to the level of parents 'Zirong' is 0, only a few is same or close to parents 'Shuijing, character between two parents but favored 'Shuijing' plant is 0, accounted for 90% of the total between two parents but favored 'Zirong'. With 'Shuijing' as the female parent descendant F2 populations, plant trait same to the level of parents 'Zirong' is 1, plant trait between two parents but favored 'Zirong' is 8, between two parents but favored 'Shuijing' plant is 36, same to 'Shuijing' have 55 strains,115 strains close to 'Shuijing' traits, the total account for around 80%of the total. This shows that 'Zirong' as female parent, characters of F1 is full of purple, characters of F2 appear separation, but most still purple, the number of green plant have no certain proportion; With 'Shuijing' as the female parent, characters of F1 is almost full of green, characters of F2 appear separation, green majority, and no separation ratio. Therefore, it can be tentatively concluded that purple and green properties of pepper materials in this study are greatly influenced by their mothers, belongs to the cytoplasmic inheritance. |
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