Application Of SSR Maker On Melon("Red Moon" Breed)Seeds Purity And Reality Identification

With the continuous improvement of industry and the active of introduction breeding work in melon, melon has been an important economic adjustment crops, but also one of the major crops in Xinjiang. To solve the problems of seed production and cultivation of regional jet lag, a fast, efficient seed identification system is necessary.In this study, a new sterile cultivar "Red Moon" melon as test materials, explore SSR markers in the melon seed purity. The following conclusions:(1) In order to determine a sett of SSR core primers fitting for melon seed purity identification, melon were analyzed by 20 SSR primers. "CMN22-22", "MU9717", "CMBR026", "CMGA128", "MU7194" and "CMBR139" were determined according to their commodity amplification products have stable polymorphism by DNA extraction, PCR amplification and electrophoresis. Which, "CMBR026" primers results clear bands, female amplified low band, high-band amplified male, F1 took the double with a polymorphism significantly, as "Red Moon" melon seed purity’s core SSR primers markers.(2) The use of "field observation" and "SSR markers" two kinds of seed purity testing methods for "Red Moon" melon purity of F1 seeds were identified, the results show:new foster production of "Red Moon" melon F1 seeds have higher purity than others. Using "field observation" and "SSR markers" identification, F1 seeds purity were 99.5% and 98.3% respectively; SSR marker identification method is superior planting observation method can accurately identify "Red Moon" F1 generation and their parents between genetic differences. The SSR maker identification way based on the primer "CMBR026" was more accurate and efficient to identify seed purity than field observation way.(3) We screen out of an core primers "CMBR026".It is impossible to stdy genome-wide genetic variation analysis of all sites in our existing hybrids for any one laboratory. In this study, we identified only six points, one point per allele is also just 2 to 4.In practical, if two test materials have the same fingerprints, we can continue to screen new SSR markers to complement and extend the preliminary established fingerprints.