The Measuring System Of Arry Culture Based On DSP

Microbial culture is a widely used experimental technique for basic research and clinical research in the field of bioengineering widely used. As the culturing process is quite long, and it involves the microbial structure, function and the interaction with the environment, the culturing process need strictly controlling of the environmental parameters and dynamic parameters. These parameters include temperature, humidity, light intensity, OD dissolved oxygen value(the OD dissolved oxygen value reflects the ventilator efficiency and oxygen transferring efficiency), rotational speed and amplitude(the amplitude influences mixing effects of the microbes and the culture medium), as well as the dynamic balancing, which will influence the stability of the culture system. Because the structural constraints of the high-throughput arrays culture system, the majority of products on the market currently lack high precision measurements for this kind of culture and the actual measured values are far below the nominal value. And the OD measurement can only be accomplished offline, dynamic balancing is also seldom considered. These factors have greatly affected the practical application of cultures in high disturbance states.To this end, this paper focuses on the on-line measuring methods of environmental parameters and dynamic parameters of the high-throughput array culture in high disturbance state. The main focus of the paper are functional requirements analysis, measuring methods design, high-precision measuring circuit design of all the parameters, relevant software development of the measuring systems and the measuring process and data analysis of array cultures with this measuring systems.First of all, by analyzing the measuring demand of the microbial array cultures, the measuring circuits related to the measuring systems are designed and developed, according to the specific requirements of the measuring range and measuring accuracy, including sensor selection and conditioning circuit design. And according to the actual need, a testing bed and testing chamber are built to simulate the actual operating environment of the culture system. According to the actual measuring conditions, the single balancing algorithm is selected to measure dynamic balance. And in order to reduce the impact caused by spectral leakage and reduce the computation amount of F2808 chip, the acceleration signals' are extracted by using the entire sample period.Then a HIM system based on LabVIEW is developed according to the actual measurement conditions and requirements, which can achieve the real-time dynamic display of temperature, humidity, light intensity, rotational speed, amplitude and dynamic balancing. The HIM system can also automatically store and replay historical data.Finally, the parameters are measured and the data is analyzed within the prototype array culture developed by the research group. The non-uniformity of the temperature and light intensity, the humidity in the culture, and changes in rotational speed, amplitude and the dynamic balancing are measured separately. Through the analysis of the measuring, it can help to understand the reasons causing these changes and provide a basis for follow-up to improve the design.The parameter measuring system of high-throughput array culture in this paper can improve the microbial culturing quality. It also has certain reference value of environmental and dynamic parameters' measuring in other occasions. At the same time, it also has some help in prompting the development of tissue culturing and the microbiology.