Fabrication Of Protein-imprinted Nonaparticles By Combining Metal Coordination With An Aqueous Precipitation Polymerization Strategy

Molecular imprinting technology is a kind of separation technology.It constructs the polymer who is around the template and moves the template through a certain way,then it will form the holes which can recognise the protein.Small molecular imprinting has been able to achieve good effect.As for protein imprinting,this method is cheap and can be reused,and has a good recognition performance of template protein,so protein imprinting technology expected to be the most useful way to construct protein separation materials and has been widely researched.However,it has been challenged by difficulties such as restricted biomacromolecule transfer in the cross-linked polymer networks,and reduced template-monomer interactions that are due to the required aqueous media.So much work have to be done to improve the imprinting performance.Faced with this problems,we mainly studied the following two aspects.On the one hand,we have imprinted the histidine(His)–exposed proteins by combining surface imprinting over nanostructures,utilization of metal coordination interactions,and adoption of aqueous precipitation polymerization capable of forming reversible physical crosslinks.With lysozyme as a model template bearing His residues and modified with acrylamide and double bond of nanoparticles as the carrier,imprinted polymer nanoshells were grafted over the carrier by aqueous precipitation copolymerization of a Cu2+chelating monomer with a temperature-responsive monomer carried out at 37 °C,above the volume phase-transition temperature(VPTT)of the final copolymer.Imprinted polymer nanoshells showed the obvious temperature sensitivity,and the template can be removel by swelling of the imprinted layers at 4 °C which is below the VPTT.The resultant core-shell imprinted nanoparticles exhibited strikingly high rebinding selectivity against the other nontemplate proteins.It achievd the best imprinting factor among the previous report which is up to 22.7 and achieved a rather high specific binding capacity of 67.3 mg/g.Moreover,this approach was extended to impingting the hemoglobin which bearing His residues and the resultant imprinted nanoparticle showed the excellent impinting performance.On the other hand,we using the aqueous precipitation polymerization method to imprint the lysozyme by pre-assembling the template to the nanoparticles though the coordination interactions.A lot of lysozyme can be fixed on the surface of nanaparticles by the means of pre-assembling,and the polymerization was induced at 37 °C.It showed a high specific binding capacity of 105 mg/g among the different modified nanoparticles.It has a much higher specific binding capacity compared with the above research,so the pre-assemble process can improve the specific binding capacity.It has a faster adsorption rate of 10 min due to the strong affinity between the nanoparticles and template through the enough coordination interactions.