Key Techniques Of A Temprature Control System For Nucleic Acid Amplication

Nucleic acid amplification is a technology that is a gene in vitro separation technology,widely used in molecular biology of basic research and application analysis,such as clinical diagnosis,infectious disease diagnosis,gene cloning and industrial quality control and other fields.Nucleic acid amplification technology used in basic research,to achieve the purpose of amplification of the target gene and test recombinants,gene cloning,gene function and expression regulation research,genome sequencing,preparation of single-strand template,mutagenicity and other purposes;nucleic acid amplification applications In clinical practice,can be applied to the diagnosis and treatment of genetic diseases,tumor research,pathogen detection,genotyping,etc.;nucleic acid amplification can also be used in the field of forensic identification.In this paper,we mainly study the thermostatic reaction device with the development of nucleic acid thermostat technology.The original temperature of the thermostatic bath device is relatively low,and the temperature distribution of the different reaction tank is different.The design and temperature uniformity of the improvement,designed to ensure a smooth progress of nucleic acid amplification device bath device.In this paper,the hardware circuit of the system is designed,the algorithm of temperature control is studied,and the finite element analysis of the reaction tank is carried out.The temperature uniformity of the reaction tank is improved by improving the reaction tank.The main contents are:(1)Hardware circuit,variable temperature module design.The system consist of the temperature acquisition module,the control chip and the peripheral circuit,the temperature change module and the auxiliary part are designed and made to build the metal bath system hardware device.(2)Temperature control algorithm design and PID parameter tuning.In this paper,the classical PID control method is adopted,and the method of relay self-tuning is adopted for the selection of PID parameters.Then,the fuzzy control method is proposed by using the given feedforward compensation control and fuzzy control method.By selecting different control methods,the performance of the system temperature control is improved.Finally,we use the final simulation method of each tuning method through Matlab to judge the tuning effect and get the optimal parameters of PID control.(3)Metal reaction tank to improve the uniformity of heat.Nucleic acid constant temperature amplification process will be the reaction tube placed in the metal tank,the reaction tank to play the role of heat transfer,the current PCR and metal bath thermostat device in many cases only take into account the temperature control accuracy and cooling rate,but no Considering the problem of temperature uniformity,in this paper,the finite element analysis method is adopted to reduce the heat exchange between the sidewalls of the sample trough and the ambient air by adding the insulating layer on the side wall of the metal trough.Improve,improve the temperature control uniformity,and ultimately improve the reliability of experimental results.(4)Experimental results validation.Through the use of multi-channel temperature acquisition system,the temperature control process temperature data collection,through the temperature control process temperature changes in the analysis,verify the system design and temperature control uniformity of the problem.Through the rapid detection of Candida,the fluorescence observation of each test sample,to determine the experimental results,verify the design of the device can be used for nucleic acid thermostat amplification experimental study.