Analysis Of SlNAC10 Transgenic Arabidopsis's Resistance And Mechanism

NAC(NAM,ATAF1/2 and CUC2)proteins were plant-specific superfamily of transcription factor,which was found in the plant widely.Its functions not only participate in plant growth and development,but also respond to biological and abiotic stress.Preliminary work in our laboratory has cloned a SlNAC10 gene from Suaeda liaotungensis,and has obtained the seeds of homozygous Arabidopsis.In this paper,we investigated the role of Sl NAC10 in response to abiotic stress(salt,drought and low temperature)by transgenic Arabidopsis.Analyzed salt,drought and cold resistance of Arabidopsis,we found that Sl NAC10 could improve the resistance of transgenic plants.We predicted it's downstream genes at the same time.Under abiotic stress(salt,drought and low temperature),morphological and physiological characteristics of transgenic Arabidopsis and wild were analyzed.Transgenic Arabidopsis showed obvious advantages in seed germination rate and root development under salt and drought.The stomatal opening rate,photosynthetic activity and survival rate of transgenic Arabidopsis were higher than the control under salt,drought and low temperature.The proline content of transgenic Arabidopsis increased rapidly under salt,drought and low temperature conditions.The betaine was accumulated rapidly under salt and drought stress,which could balance the ions of the solute.Transgenic Arabidopsis had higher activities of SOD and POD under salt,drought and low temperature.CAT has higher activity under salt and drought.These results indicated that SlNAC10 enhances the resistance of transgenic Arabidopsis.Gene expression of transgenic Arabidopsis was analyzed by gene chip technique.We found genes that regulate seed germination,root development and photosynthetic activity.Seed germination,root development and photosynthetic activity of transgenic Arabidopsis was increased,which unified the results of morphological and molecular.At the same time,useing gene chip technology has found a large number of genes expressed differentially.They respond to osmotic stress,oxidative stress,hormone stimulation and other abiotic stress enrichment.They related to arginine and proline metabolism,indole alkaloid synthesis,plant hormone signal transduction,glutathione metabolism and other related metabolic pathways,which can play an important role in plant resistance to abiotic stress.In summary,SlNAC10 gene significantly enhanced salt tolerance and drought resistance,cold resistance also increased to varying degrees.It can be considered that the SlNAC10 gene plays an important role in the plant's response to stress.It enhanced plant resistance by regulating other downstream genes.We hoped the results of this study will help to improve the function of NAC and its downstream network.