| Villin is one of the actin binding protein familys. It plays an important role in regulatingactin dynamics through making actin filament bundled, capped, cut, nucleationHowever, the function about Villin in plant is little knowen. In this study, we analyzedthe expression patterns of the five VLN genes (VLN1-5) in Arabidopsis, the expressionchanges of VLN1and VLN5under salt and oxidative stress. Morever, we investigatedphenotypes under salt and oxidative stress, ROS level, and microfilament dynamics of VLN1and VLN5mutants. The results were as follows:(1) Arabidopsis Villins were expressed ubiquitously in young tissue and leaf, but therewere many differences in root and flowers. Specially, VLN1and VLN5were highly expressedin root and gard cells.(2) The expression level of VLN1and VLN5were increased under salt and oxidativestress. We found that the expression of VLN1and VLN5in14-day-old wild type seedlingsunder treatmeant for3h,6h and12h were both induced by NaCl (150mM) and MV (0.5μM).The expression level of VLN1and VLN5peaked at12h treated by NaCl and at3h stressed byMV.(3) The germination and growth of VLN1and VLN5mutants are impact by salt anddrought stress. The germination rate of VLN1and VLN5mutants is lower than the wild typeunder the stress of150mM NaCl. And the drought resistance of VLN1and VLN5mutants isalso lower than the wild type.(4) ROS level of VLN1and VLN5mutants were turn to high under stress.(5) The dynamics of actin filaments in VLN1and VLN5mutants under stress weredifferent from WT. The actin filaments localization and the bundle changed in the maturationof root in VLN1and VLN5mutants compared with that of the wild type. And the actinfilaments were cut into small fragments in hypocotyl of VLN5mutant. The depolymerizationof actin filaments in the guard cell of VLN1and VLN5mutants are far higher than the widetype under the stress of LatB and MV. This result suggested that VLN1and VLN5may be involved in actin dynamic regulation and steadied the actin filaments in vivo. |
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