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The Morphologic Observation,Autecological Studies And Sequences Analyses For Four Red Algae In Gigartinales

Posted on:2017-06-09Degree:MasterType:Thesis
Country:ChinaCandidate:P JiangFull Text:PDF
GTID:2310330488968786Subject:Cell biology
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A research of Grateloupia subpectinata Holmes which is collected in Zhejiang,Guangdong,Liaoning province on the basis of molecular and morphological data.Results indicated that:(1)thallus was dark purplish red,Single or cluster,reach a height of 15–50 cm,fleshy in texture,becoming somewhat cartilaginous when old,the branches become covered with proliferations and be pinnately arranged;(2)the auxiliary cell ampullae and carpogonial branch ampullae exhibited typical Grateloupia-type,carpogonial branch ampullae consisted of 5 cells,auxiliary cell ampullae consisted of 4 cells(5cap-4auxb type);(3)the ribulose-1,5-bisphosphate carboxylase/oxygenase gene(rbc L)sequences of eight G.subpectinata from Zhejiang,Guangdong province and dalian country examined showed that there was no intergeneric divergence among them,there is 1bp(0.08%)intergeneric divergence between the eight specimens and G.subpectinata collected from Korea and Japan;(4)TheUPA sequences of eight G.subpectinata from Zhejiang,Guangdong province and dalian country examined showed that there was no intergeneric divergence among them,there is 1bp(0.29%)intergeneric divergence between the eight specimens and G.subpectinata collected from Korea;(5)Through the morphological observation and gene analysis that we concluded that the red algae of our research is the same species of G.subpectinata is identified as a new record species in China.Morphological observations supported G.subpectinata and G.didymecladia which was named a new species in Flora Algarum Marinarum Sinicarum as the same species,we regard G.didymecladia as synonym of G.subpectinata.A research of Dumontia simplex Cotton which is collected in Dalian country on the basis of morphological,Autecological studies and molecular data.Results indicated that:(1)The size and morphology of each individual plant collected in different Location are different;(2)The size of mature cystocarp is small and mature tetrasporangia is big;(3)The individual begin to grow in Decemberand the biomass is minimum,the individual is mature in February and disappeared in June;(4)The main mature season of D.simplex is from February to May;(5)the rbcL,COI and UPA sequences of D.simplex from different Location in Dalian country examined showed that there was no intergeneric divergence among them.A research of Hyalosiphonia caespitosa Okamura which is collected in Dalian country on the basis of morphological,Autecological studies and molecular data.Results indicated that:(1)The size and morphology of each individual plant collected in different Location are different.(2)The size of mature cystocarp and mature tetrasporangia are big.(3)The individual begin to grow in December and the biomass is minimum,the individual is mature in March and disappeared in July.(4)The main mature season of H.caespitosa is from April to July.(5)The rbcL,COI and UPA sequences of H.caespitosa from different Location in Dalian country examined showed that there was no intergeneric divergence among them.A research of Gloiosiphonia capillaris(Hudson Carmichael)which is collected in Dalian country on the basis of morphological,Autecological studies and molecular data.Results indicated that:(1)The size and morphology of each individual plant collected in different Location are different.(2)The size of mature cystocarp and mature tetrasporangia are big and scattered arrangement.(3)The individual begin to grow in March and the biomass is minimum,the individual is mature in May and disappeared in October.(4)The main mature season of G.capillaris is from June to September.(5)The rbcL,COI and UPA sequences of G.capillaris from different Location in Dalian country examined showed that there was no intergeneric divergence among them.
Keywords/Search Tags:Grateloupia subpectinata, Grareloupia didymecladia, Dumontia simplex, Hyalosiphonia caespitosa, Gloiosiphonia capillaris, Autecological studies, Morphological observation, Molecular analysis
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