| Eustoma grandiflorum is favored by the consumers, but it demands environmentsuch as soft light, warm tempriture and suitable air humidity expecially, and issensitive to antibiotic.Antibiotic stress makes the ROS burst, which damages the cell structure andprotein system. Carotenoids are non-enzymatic antioxidants, playing an importantrole in plants, which have a great impact on the plant tolerance to oxidation verymuch. To enhance the tolerance to antibiotic stress of Eustoma Lisianthus, weincreased the accumulation of the carotenoid biosynthesis in Eustoma grandiflorumShinn by overexpressing ?-carotene hydroxylase gene (LcCHYB) from LyciumChinense encoding ?-carotene hydroxylase (BCH) and treat the plants by10%hydrogen peroxide for20min. This enzyme catalyzes the reaction of ?-carotene tozeaxanthin and plays an important role in the carotenoid biosynthesis. The results areas follows:1Optimization of the the medium used in the transformation of E.grandiflorum S.. In this research, we optimized the rooting medium and determinedthe kanamycin concentration for screening and the cefotaxime concentration forinhibition of Agrobacterium.2The expression of LcCHYB enhanced the carotenoids biosynthesis. Thecontents of total carotenoids, zeaxanthin and the Xanthophyll cycle are up-regulated,whose value are18.51?g/g?6.28?g/g and13.10?g/g, respectively.3The value of photosynthetic fluorescence parameters of transgenic line arehigher than the wild line.4The value of SOD and POD of transgenic line are higher than the wild line,and up-regulated when treat by the10%hydrogen peroxide for20min.Our results indicates that the overexpression of LcCHYB enhances the toleranceto oxidative stress and prevents the ROS damaging the cell to enhance the tolerance toantibiotic stress... |
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