| Carotenoids play an important role in the whole life of plants. In order to studythe relationship of carotenoids and salt tolerance of plants,we transfer the key enzymegene in carotenoid biosythesis into Eustoma grandiflorum byAgrobacterium-mediatedtransformation obtain three lines with high expression levels.The transgenemanipulation leads to1.27fold increase of total carotenoids. Non transgenic plants(WT) and transgenic lines were treated with200mmol/L NaCl, found that thetransgenic lines had higher photosynthetic rate and photochemical efficiency andlower MDA level.Under salt stress,the transgenic plants maintained higher SOD andPOD activity, indicating that the exogenous gene LcPSY expression protected plants;In conclusion,LcPSY is probably a promising candidate for salt improvement in crops.Jasmonic acid is considered to be signals of plant response to biotic and abioticstress gene expression change. Allene oxide cyclase (AOC) gene is a key gene injasmonic acid biosynthesis pathway. We studied of this gene in response to abioticstress to provide evidence for Lycium chinense jasmonic acid synthesis pathway.Weisolated AOC gene from the Lycium chinense the first time. Sequence analysis showedthat full-length cDNA of LcAOC is1092bp, the complete open readingframe(ORF)of this gene is744bp, encoding for a protein of247amino acids with anisoelectric point of8.22and a molecular weight of26.9kD. The predicted proteinsubcellular localization in chloroplasts. By bioinformatics to analyze LcAOC aminoacid sequence we found that LcAOC protein is a non-transmembrane andhydrophilic protein. LcAOC gene expression have improved significantly AfterLycium chinense Miller suffered4℃and MeJA stress. |
