Sunset Yellow FCF And Amaranth Molecularly Imprinted Sensor Research And Its Application

In recent years, there are many news report that food pigment (such as the sunset yellow and amaranth, etc.) caused unhealth effects to human body because of excessivly or prohibitedly used. The traditional laboratory detection method can not meet the demand of the rapid detection of food. So it is urgently to develop a convenient and accurate detection method to the supervision of the use of pigment. Molecular imprinting (MIP) sensor attracts wide public concern because of its high sensitivity, high selectivity and good stability. In this paper three kinds of MIP electrochemical sensor were successful preparation combining molecular imprinting technique and electrochemical detection technologies, which was used in food pigment fast detection.(1) Sunset yellow and amaranth MIP electrochemical sensor were prepared using o-phenylenediamine as polymerization monomer and sunset yellow and amaranth as template molecules respectively, which polymerized directly on the glassy carbon electrode surface using cyclic voltammetry method, then eluted template molecules using anhydrous ethanol. The tow sensors rapid detected template molecules showed good recognition and reproducibility. Sunset yellow MIP sensors has a good linear relationship in the range of 0.0002 g/L～0.001 g/L realizing rapid detection of sunset yellow in drinks. Amaranth MIP sensors has a good linear relationship in the range of 0.005 g/L～0.05 g/L realizing rapid detection of amaranth in wine.(2) Another sunset yellow electrochemical sensor was prepared through electric polymerization, which using pyrrole as polymerization monomer and sunset yellow as template molecule, removing template molecules in 0.1mol/L NaOH solution and constant potential condition. It had a good linear relationship in the range of 0.0004 mmol/L and 0.002 mmol/L realizing rapid detection of sunset yellow in wine.(3) Compared the electrochemical performance of the two sunset yellow molecularly imprinted sensors which were used o-phenylenediamine and pyrrole as polymeric material, it is found that the sensitivity of MIP electrode which used pyrrole as polymeric material was higher and the initial peak current up to 150μA, and o-phenylenediamine material electrode was only 115μA. At the same time polypyrrole MIP electrode reproducibility was better, for it was continuously used 8 times the current response of the initial value was 90.74%, and the o-phenylenediamine MIP electrode continuously used 6 times the current response of the initial value was only 71.11%.