Research Of Molecular Imprinted Electrochemical Sensor For Paracetamol

Paracetamol(PR) is one of the world's most widely used nonprescription medicines, due to its analgesic's and antipyretic's properties. However, overdoses of PR cause adverse reaction and liver damage. In this study, three kinds of PR electrochemical sensors were constructed based on molecular imprinting technology and electrochemistry analysis technology. The main work of this thesis is as follows:①A new molecularly imprinted polymer(MIP) sensor for PR was fabricated by electropolymerization in this work, with PR as template molecule and o-phenylenediamine(o-PD) as monomer. The K₃Fe(CN)₆ was used as a molecular probe, and the sensor exhibited good selectivity and sensitivity to PR. The square wave voltammetry response of potassium ferricyanide was linear to the concentration of PR in the range of 2.0×10^-68.0×10^-5mol/L, with a detection limit of 2.0×10^-8mol/L. The proposed sensor was applied to determination of PR in different tablets and the obtained results were satisfactory.②Molecularly imprinted polymer modified glassy carbon electrode(MIP/GCE) was prepared by electropolymerization of equimolar resorcinol and o-PD on glassy carbon electrode in the presence of PR as the template. The property of MIP/GCE is investigated by cyclic voltammetry and AC impedance. And the response properties of different electrode in the same concentration of PR is investigated by cyclic voltammetry, it was found that the response current of the MIP/GCE prepared by dual-monomer is larger than which prepared by a single monomer. Square wave voltammetry was used to the direct detection of PR on the MIP/GCE.③A novel MIP sensor was constructed for detection of PR. The sensitive layer was synthesized on the multiwall carbon nanotubes modified glassy carbon electrode (MWNTs/GCE) with PR molecule as a template and o-PD as monomer. The results showed that MIP/MWNTs/GCE had an obvious electrocatalytic effect on PR. The square wave voltammetry response of PR was linear to its concentration in the range from 2.0×10^-6 to 8.0×10^-5mol/L (R2=0.9993), with a detection limit of 1.0×10^-8mol/L. The sensor exhibited a good stability and reproducibility.