| Electrochemical luminescence(ECL) is a novel detection technology with some advantages, such as high sensitivity and specificity, low time-consuming, simple apparatus and low cost, which have attracted a lot of attention. Tris(2,2’-bipyridyl)ruthenium [Ru(bpy)32+] is one of the most widely used electrochemical luminescence reagent. Compared with other reagents, it has good optical signal stability, high quantum yield, wide application range, satisfying reusability and so on. Histamine is a kind of autologous active substance. A few amount of histamine in the body can regulate blood pressure, control neural activity, and has certain advantages for the body. But a certain amount of histamine will cause harm to the human body, make the body poisoning. Diethylstilbestrol(DES) is a synthetic nonsteroidal environmental estrogen. But DES is harmful to the human body, so the National Department of Agriculture prohibit the addition of DES in fodder. But for the sake of economic interest, DES is still used illegally.This article briefly described the principles of electrochemical luminescence and the application research progress, which mainly explained immobilize and labeled method of Ru(bpy)32+. Further, the research carried out in two parts, one was to establish an electrochemical luminescence sensors to detect histamine, and the second was to set up an electrochemiluminescence immunosensor of DES. The standard curve was I=326.7lgC+4917.9 and ΔI=1537.36lgC+11094.71. The detection range of 1×10-9~1×10-5g/ml and 5×10-8~5×10-2mg/ml for histamine and DES, respectively. In the first part of the experiment, four different composite films of MWCNTs/TiO2/Nafion, MWCNTs/ZnO/Nafion, MWCNTs/SiO2/Nafion and MWCNTs/Fe3O4/Nafion were prepared, which were used to modify glassy carbon electrode. Electrical signals and optical signals were detected in order to determine the best metal oxides. The effects on detection signal of the concentration of MWCNTs in compound membrane, pH of the solution, scan rate and photomultiplier tube were studied. The conclusion obtained were as follows:(1) The electrical and optical signals were the best when modified by MWCNTs/ZnO/Nafion composite film on glassy carbon electrode,(2) The addition of MWCNTs in compound membrane enhanced the electrical signals, with the increase of redox peak current rise and decrease of redox potential difference,(3) The optimal experimental conditions: MWCNTs concentration in MWCNTs/ZnO/Nafion compound membrane were 1.00 mg/ml, the pH of solution was 7.8, the scanning rate and the photomultiplier tube voltage are 100 mV/s and 900 V. In the second part of the experiment, RuSiO2 nanoparticles were prepared of and antibodies was labelled successfully and sensor preparation was characterized by cyclic voltammetry. The activation time of carboxylic MWCNTs, the incubation time of diethylstilbestrol complete antigen and RuSiO2-Ab1 incubation time, as well as the stability and anti-jamming ability of the sensor were verified. The conclusion were as follows:(1) The RuSiO2 nanoparticles prepared has obvious core-shell structure under the transmission electron microscopy(TEM), has obvious core-shell structure, good dispersibility, size uniformity, with 50 nm particle size, size uniformity, good dispersibility and stable fluorescence.(2) The change of the cyclic voltammetric curve in the process of electrode preparation indicated substance have modified on the surface of the electrode successfully.(3) The activation time of carboxylic MWCNTs, incubation time of diethylstilbestrol complete antigen and RuSiO2-Ab1 incubation time were 40 min, 40 min and 30 min, respectively.(4) The sensor showed the long-term stability, and could not be interfered by other substances. |
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