A Fluorescent Nanothermometery Based On [Ru（Bpy）₃]²⁺-doped Silica Nanoparticles:Synthesis And Temperature Sensing Properties

Temperature is probably the most fundamental physical parameter in all kinds of system. Silica nanoparticles doped with [Ru(bpy)3]2+a kind of luminescent temperature probe were prepared via advanced Stober method and can show the ambient temperature changes by its luminescent intensity. Thus it is important to obtain temperature information of systems for knowing the state and operating the systems. Equally, for biosystem, temperature is a key parameter. In the minimum bio-unit:cells, temperature fluctuation affects many cell metabolic activities, protein synthesis, gene expression, and cell respiration, for instance. Thereby, it is important for analyzing cell activities to obtain intracellular temperature in real time. Moreover, the temperature of diseased cells is higher than the normal cells, like cancer. Thus, it is also significant for cancer diagnosis and therapy to measure temperature of tumor cells. In this article, we plan to synthesize fluorescence thermometry for intracellular temperature mapping.The main task of this research are:1. Synthesizing silica nanoparticles which have small size (<100nm) and are monodisperse by using advanced Stober method, based on which [Ru(bpy)3]2+doped silica nanoparticles are synthesized. The [Ru(bpy)3]2+@Si02nanoparticles will be surface modified with Poly-L-Lysine to promote its biocompatibility.2. Based on the luminescence of [Ru(bpy)3]2+, a series of temperature sensing experiments were performed to obtain the sensitivity data to temperature of [Ru(bpy)3]2+@SiO2nanoparticles. And HepG2cells were incubated ([Ru(bpy)3]2+@SiO2)@PLL nanoparticles and gold nanorods modified by mPEG5000-SH to map intracellular temperature changes.Synthesizing silica-coated gold nanorods with different thickness of silica layer for further research of enhanced fluorescence doped nanoparticles to enhance the sensitivity of fluorescence doped temperature nanosensor.