The Effect Of Flavonoids Puhuang Estrogen-like On The Uterus Of Adult Female Rate

Objectove:Research on flaconoids Puhuang estrogen-like effects on the uterus of adult female rats the effects of local circumstancesMethod:SD rats(n=60) were randomly divided into 5groups:normal group(n= 12),Tamoxifen+Puhuang flavonoid guorp(n=12),Phuang flavonoid group(n=12),Tamoxifen group(n= 12).Administration of oral gavage once daily administration for 21 days,every five days during the administration of said weight and do a good job in record time.5days before administration of vaginal exfoliated cells from picture search,the first day,according to vaginal smear exfoliated cells of rats to determine the cycle period beginning on the proliferation of drug delivery.Administration after the end of the first day of the execution,the execution of rat carotid artery after collecting serum from serum E2 and P levels.Bilateral middle cut the uterus,weighetde into 4% paraformaldehyde fixation solution,and histopathological and immunohistochemical examination,measuring ER-α,ER-β,and P the average gray value and the average optical density value express their positive cells.Result:And I compared Puhuang flavonoid in rat endomertrial ER-a,ER-βand expression of P-positive cells and blood content of E2 and P were significantly increased (P< 0.01); compared with group II,Puhuang flavonoids in rat endometrial ER-a,ER-βand the expression of P-positive cells and the blood content of E2 and P were increased significantly increased(P<0.01);and 4 group, flavononodis Puhuang rats in endometrial ER-a,ER-(3 and the expression of P-positive cells and the blood content of E2 and P had no statistical significant change was not significant (P>0.05).Conclusion:Puhuang flavonoids with estrogen-like effects in rats can significantly improve endometrial ER-α,ER-βand the expression of P-positive cells and the blood content of E2 and P at the same time can promote the development of the uterus.