The Effect Of Different Wavelengths Of Monochromatic Lights And Their Combination On Refractive Development In Guinea Pigs

OBJECTIVETo study the effect of different wavelengths of monochromatic lights and their various combinations on refractive development in guinea pigs,exploring the difference of refraction on different degrees of color imbalance.METHODS40 healthy guinea pigs were randomly divided into 8 groups(n=6),respectively feeded in eight kinds of light environment: Blue Light(BL),Green Light(GL), Red Light(RL), Blue+Green Lights(B+G), Red+Green Lights(R+G), Blue+Red Lights(B+R), Blue+Red+Green Lights(B+R+G),and White Light(WL) as control group. Blue, green and red lights respectively corresponded to a certain wavelength. Specially designed feeding boxes were independent to each other, and the size was about60*40*40cm. A set of LED lights was respectively installed on the twoends of the inner part of each box, covered in a transparent lampshade,which formed diffuse lights. The brightness in each box was controlled the same as 80cd/m2. The lights of each group circulated as12/12h(8:00 on, 20:00 off), maintaining consistency all the time. The experimental period was 12 weeks. Two eyes of all guinea pigs were measured with the refractive index and the axial length one day before the experiment, then measured every two weeks. The measurement data to statistical analysis was all from the right eye of each guinea pig.RESULTS1 Changes in refraction1.1 The general refraction of each group after 12 weeks(the figures below for the refraction, iu D)RL <0< G+R < GL < B+G ≈ B+G+R ≈ WL <B+R< BL-2.42±0.408 0.5.00±0.570 2.00±0.387 3.13±0.262 3.08±0.258 3.29±0.368 5.04±0.714 6.25±0.3161.2 The degree of changes of the refraction before and after the experiment for each group(that is, the V1 value, the figures below for the degree of refraction, iu D)The dgree of myopization : RL>G+R≈ GL>B+G≈B+G+R≈WL-6.63±0.345-3.96±0.660-3.42±0.719-2.29±1.123 0.94±0.765-1.42±0.736Among them, B+G>WL, difference exists(P<0.05)The dgree of hyperopization: BL > RL1.79±0.714 0.83±0.0.3761.3 The time sequence of the refraction of each group starts to appear different with the white light control group:Myopization: RL(2 weeks)â†'G+R(4 weeks)â†' RL(6 weeks)Hyperopization: BL(4 weeks) â†'B+R(6 weeks)There was no difference in refraction between B+G and WL,B+G+R and WL.2 Changes in the axial length2.1 The general axial length of each group after 12 weeks(the figures below for the axial length, unit mm):RL > G+R > GL > B+G≈ WL ≈B+G+R > B+R > BL8.93±0.052 8.79±0.031 8.62±0.044 8.45±0.044 8.45±0.043 8.41±0.031 8.38±0.045 8.31±0.0382.2 The degree of changes of axial length before and after the experiment for each group(that is, the V2 value, the figures below for the degree of axial length, iu mm)RL > G+R > GL > B+G ≈ WL≈ B+G+R≈ B+R ≈ BL1.51±0.111 1.34±0.113 1.19±0.090 1.03±0.070 0.98±0.100 0.94±0.091 0.88±0.066 0.82±0.073Among them, B+G>B+R, significant difference exists(P<0.01)B+G+R>BL, difference exists(P<0.05)2.3 The time sequence of the axial length of each group starts to appear different with the white light control group:RL, G+R, GL, BL(4 weeks) â†' B+R(6 weeks)No difference exists between B+R, B+G+R and WL, always.conclusion1 Long-wavelength monochromatic light induced myopia, while short-wavelength monochromatic light induced hyperopia in guinea pigs.2 Different combinations of monochromatic lights caused different degrees of color imbalance, inducing different degrees of refractive changes in guinea pigs, but not each combination caused difference.3 The refractive changes induced by different combinations of monochromatic lights in the guinea pigs, were not just towards the trend of mean wavelength, and the time of the refraction and the axial length of each group started to appear different with the white light control group was different. In general, long-wavelength monochromatic light induced refractive changes earlier, faster and more..4 The color-imbalance myopia of guinea pig was probably regulated by M and S cone cells, and the regulation of M and S cone cells was probably bidirectional.5 When both M and S cone cells were suitably stimulated the most,the regulation of the two may be in balance and not easy to be broken by some other factors.