Study Of The Association Between The Rs10754558 And Rs2027432 Olymorphisms In NLRP3 And The Genetic Susceptibility Of Preeclampsia In Chinese Han Population

Objective The purpose of our study was to detect the distribution situations of the single nucleotide polymorphism(SNP)s, rs10754558 and rs2027432, in NLRP3 and to investigate the association between the two SNPs and the genetic susceptility of preeclamsia(PE) in Chinese Han population.Methods The NLRP3 polymorphisms, rs10754558 and rs2027432, were genotyped by real-time PCR in 1024 PE patients and 1194 control subjects. A χ2 test was used to compare the genetic distribution and allele friquency between the two groups, and an analysis of variance was used to conduct the genotype-phenotype analysis.Results We demonstrated a significant difference in genotypic frequency of the rs10754558(χ2 =9.97, P =0.007) in NLRP3 between cases and controls. Additionally,the genotypes distributions of rs10754558 were found to be associated with both the severe and late onset PE.( For severe PE:χ2=8.53 P=0.01; For late onset PE: χ2=9.24,P=0.01). However, no significant statistic differences were found in the genotypic distributions and allelic frequencies for rs2027432 between two groups(For genotypic distribution, χ2=0.17,P=0.92;For allelic frequency, χ2=2.26,P=0.13,odds ratio =0.90, 95% confidence interval0.79-1.03).Conclusion Our results reveal that the rs10754558 polymorphism of NLRP3 may be involved in the development of PE in a Chinese Han population.But we failed to find that rs2027432 was associated with the development of PE.