The Antitumor Activity Research Of The Ethanol Extract From Fructus Schisandrae Nuts

Schisandra is that people are more familiar with, can be used as health food raw materials of traditional medicine.In recent years, the ingredients for a lot of active research data, but only in relatively concentrated fruit pulp or part,there are also reported in the literature leaves the Schisandra lignans content of ingredients, but the study has not been large-scale expansion of Schisandra nuts.According to related statistics, Schisandra nuts in foreign sales market has been good, and in foreign countries also have a lot of research carried out only for Schisandra nuts.This illustrates the considerable research value Schisandra nuts potential.If you can identify their nuts active material composition and its functions will be to delve into Schisandra great significance.The raw materials used for the experiment Schisandra nuts to extract the ethanol extraction method determined by the large number of documents.Study design univariate variable experiment, by screening out the high anti-tumor activity rate of 3 factors application response surface method.Extracts of the qualitative and quantitative experiments measured by LC-MS analysis,respectively, sample and standard samples, sample processing and analysis by LC-MSn data in comparison with the standard obtained, identified the main material composition and content of the Schisandra nuts ethanol extract.By then cultured H22 hepatoma cells in vivo and S-180 sarcoma cells, the use of statistical indicators of the data confirmed the inhibition of tumor activity of ethanol extract of Schisandra nuts.Experimental results show that:1、Ethanol extraction of single factor experiment, as determined by MTT method, the tumor inhibitory rate of high and low to measure, screening the best three levels of each factor. Optimal extraction process optimization using response surface software design scheme for the material liquid ratio of 1:10, ethanol concentration was 85%, the extracting time of 4h.The best extraction conditions, fructus schisandrae nuts nethanol extract yield of 1.764%.2、Fructus schisandrae nut ethanol extract antitumor activity(1) Through animal model test, the fructus schisandrae nuts that wooden fat element on H22 liver cancer cells in mice with ideal effects. The influences of hemoglobin in mice, the inhibitory rate of tumor in mice and each dose group were ideal. Each dose group in mice tumor inhibitory rate are: Dose groups in high dose group was 48.90%, middle dose group was 31.36%, 12.43% low dose group.(2)Through animal model test, the fructus schisandrae nuts that wooden fat element restrain the activity of S-180 sarcoma cells in mice is obvious. The effect on mice hemoglobin larger influence on red blood cells at the same time is also very significant. Dose groups in high dose group was 60.37%, middle dose group was 42.58%, 32.60% low dose group.The research results indicate that Schisandra nuts ethanol extract has obvious tumor suppressor biological activity.