The Anti-obese Activity Of Hypoxanthine And The Determination In Velvet Antlers

Antler is a precious traditional Chinese medicine and it contains mineral element, peptides, polysaccharides, polyamines and other biologically active substances. Modern scientific research shows that antler plays a vital role in regulating body metabolism and promoting a variety of physiological functional activity. In China, there has been thousands of years history of velvet antler used as a traditional Chinese medicine. Owing to the velvet antler with plentiful pharmacological activity, so it is often used in clinical research.1. In this paper, it measured the content of hypoxanthine in five kinds of velvet antler by High Performance Liquid Chromatography. The results showed that the content of the alcohol extract of hypoxanthine sika velvet antler, wapiti velvet antler, hybrid antler, New Zealand antler, reindeer velvet antler, were 0.355mg/g, 0.161mg/g, 0.315mg/g, 0.159mg/g and 0.266mg/g respectively. It was better than the content of hypoxanthine in sika flower velvet antler, and it was closed to the content of hybrid antler and reindeer velvet antler. It was basically similar and related in the literature reported in the content of hypoxanthine in the above five kinds of velvet antler.2. In this paper, to study on the anti-obese activity of hypoxanthine on nutritive obesity mice.After 4 weeks of oral administration, then detect the mouse serum triglycerides, total cholesterol, leptin and adiponectin and so on. The results showed that after oral administration, administration group had varying degrees of interference in fat of mice. The weight of hypoxanthine treatment groups significantly reduced the quality of fat in obese mice than model group in mice(P<0.05, P<0.01); The fat of perirenal and abdomen was significantly decreased. The levels of total cholesterol and triglyceride were reduced in obese mice, HDL-C and LPS levels were also significantly improved(P<0.05, P<0.01). The hypoxanthine worked for low and middle dose of treatment groups. The activity of SOD was increased and the content of MDA was decreased in liver. The above experiments results showed that hypoxanthine medium and low dose group can significantly reduce fat mass in obese mice, and the effect anti-obesity of low-dose group was better than the middle dose group of hypoxanthine. It was comprehensively showed that the low and middle group dose of hypoxanthine can reduce the quality of obese mice significantly, and the effect of the low dose group of hypoxanthine in anti-obesity is the best in the experiment.3. The tissue was made paraffin section slices of abdominal adipose and liver tissue in mice. The pathological slices of mice were observed through the optical microscope. The section of obese mice abdominal adipose tissue showed the diameter of fat cell becomes larger, a dozen vacuolated, irregular shape, the phenomenon of cell fusion. The adipose tissue of mice significantly shrank, lipid droplets decreased, and fat cells became smaller, the volume of fat tissue cells appeared different degree of recovery, after the oral administration treatment of hypoxanthine. From the observation of the obese mice liver tissue slice, the midle and high groups of hypoxanthine and positive medicine group, the degree of liver cells steatosis in mice with severe unequal fatty degeneration in mice. Ultrastructure exist scattered punctate lesions in low dose group of hypoxanthine, the degree of hepatic steatosis was better after treatment.Paraffin section productiont was used, to observe the changes of cells and tissues by HE color. Which provided the basis of weight loss in obese mice for further research.4. In this paper, analysis by real-time quantitative PCR. The expression of obese gene(leptin and adiponectin) mRNA and AP-2, PPAR-γ, LPL, was used to observe the effect of hypoxanthine to lose weight. The middle-dose and low-dose groups of hypoxanthine and normal control were significant difference than the model group(P<0.05). The expression of PPAR-γ, AP-2 and LPL mRNA genes with statistical difference in middle-dose and low-dose groups of hypoxanthine than modle group(P<0.05). Thus it provided a basis for hypoxanthine in the weight recuction from the gene level.