The Significance Of DNA Ploidy And P53 Measurements In Lymph Node Micrometastases On Endometrial Carcinoma

Objective:Analysis at the relationship between endometrial carcinoma lymph node micrometastasis rate and histological grade, pathological stage, myometrial infiltration,pathological type, age, whether with hypertension or not,whether complicated with diabetes or not, body mass index. Compared metastasis lymph node negative group with expression difference of non metastatic group negative lymph node DNA,and P53 protein.To study DNA ploidy detection,P53 detection and detection of DNA ploidy combined with P53 in the diagnosis of lymph node micrometastasis on endometrial carcinoma.Provide help for clinical diagnosis and individualized treatment of endometrial cancer patients.Methods:1.32 patients with Endometrial cancer who had not received treatment before surgery were collected, and 316 lymph nodes were collected, including 250 lymph nodes without metastasis(Group 1) and and 66 negative lymph nodes(Group 2).2.Use HE staining in the diagnosis of tumor tissues and lymph nodes of patients with endometrial cancer.3.Use DNA-AICM detection system to analyze the DNA ploidy types in tumor tissues and metastatic negative lymph nodes in patients with endometrial cancer.4.Use IHC detection to analyze P53 protein in tumor tissues and metastatic lymph nodes in patients with endometrial cancer.5.Use SPSS13.0 analysis software,and statistical analysis using Chi-Square Tes-ts.Results:1. In the analysis of DNA ploidy in 32 cases of endometrial carcinoma, 21 cases(65.6%) were diploid, 11 cases(34.4%) were aneuploid. In histological grade,pathological stage, muscular layer infiltration depth and endometrial carcinoma pathological group were compared, with the same DNA aneuploid expression rate difference was statistically significant(P<0.05);In age, body mass index and whether a merger hypertension, diabetes etc groups were compared and DNA aneuploid expression difference of rate was no significant(P>0.05).2. In the analysis of P53 protein in 32 cases of endometrial cancer patients, 19 cases(59.4%) patients with P53 negative expression, 13 cases(40.6%) patients with P53 positive expression. Compares the differences among them in organizational learning grade and combined diabetes group, P53 positive expression of the difference has statistical significance(P<0.05). In endometrial carcinoma histological type, pathological stage, age, body mass index(BMI), infiltration depth of muscular layer and whether complicated with hypertension were compared within and P53 positive expression difference no statistical significance(P>0.05)3.In 316 metastasis negative lymph node, histological grade, pathologic stage,myometrial invasion depth, pathological type groups are compared.lymph node micrometastasis(P53 or DNA aneuploid positive expression rate difference was statistically significant(P<0.05); Within age, body mass index, and whether a merger hypertension, diabetes etc. groups were compared, lymph node micrometastasis rate difference was not statistically significant(P>0.05).4. By comparing the 250 non metastatic lymph nodes and 66 metastatic lymph nodes, there was a statistical significance on the difference for expression rate of DNA aneuploid and P53 positive expression in the two groups(P< 0.05).5. In 316 metastasis negative lymph node,52(16.46%) were detected withmicrometastases(P53 or DNA aneuploid was in expression positive).DNA aneuploid expression of 32(10.13%) were positive, Among them, 28 were(8.86%)P53 expression positive, 8(2.53%) were both P53 and DNA aneuploid expression were positive. There was no significant difference in the expression of DNA ploidy and P53 in the metastatic lymph nodes(P>0.0167). The combined detection of both can significantly improve the lymph node metastasis rate(P<0.0167).Conclusion:1.In pathological examination of lymph nodes without metastasis, the positive expression can be detected in DNA ploidy, P53 protein in varying degrees different,suggesting that non-metastatic lymph node biopsy may have micrometastases.2.Lymph node micrometastases of endometrial cancer have clinically significant differences between the various indicators(histological grade, surgical stage, myometrial invasion and histological type),suggesting that patients with risk factors may be more likely to occur micrometastases.3.The group of negative lymph node metastasis compared with the group of non-metastatic lymph node-negative,DNA aneuploidy and P53 expression was significantly higher, suggesting that in negative lymph node metastasis than those without lymph node metastasis negative group, it may be easier to find micrometastases.4.In the detection of lymph nodes metastasis, DNA aneuploidy and P53 protein were not different,and the combined detection can improve the rate of lymph node micrometastasis,It has some tips value for clinical follow-up and individualized treatment.