| Purpose:Through the study on the correlation between asthma in children with Helicobacter pylori infection and other environmental, social and immune factors of children with childhood asthma, in order to give some experimental basis for the prevention and treatment of asthma in children.Methodology:1. Find articles about the relationship between Asthma and Helicobacter pylori(Hp)from database,then use them to make the Meta analysis. At the same time, blood samples of 33 children with Asthma and 37 healthy children from May 2015 to Dec 2015 were selected from Taiyuan Disease Control Center. ELISA was applied to both the group of children with Asthma and the control group to analyze the antibody count of Hp-Ig G and analysis was performed to determine the statistical significance of the relationship between Hp infection and Asthma.2. Questionnaires used for analysing the possible risk factors for childhood asthma were collected from Taiyuan Disease Control Center,which conclude is answered by 46 children with Asthma and 54 healthy children and their guardians(mainly the parents).Topics on the questionnaires include the basic living conditions of the children(how they were given birth and how they are fed), children’s reactions to allergens(e.g. Animal fur,pollen or cold air), reaction to antibiotics, family history and living environment(humidity,cleanness and exposure to smoke etc.). Analysis on the relationship between children’s living conditions and Asthma was performed by analyzing the questionnaires using Epidata3.1 and SAS9.2.3. Blood samples of 33 children with Asthma and 37 healthy children from May 2015 to Dec 2015 were selected from Taiyuan Disease Control Center. Elisa was applied to both the group of children with Asthma and the control group to analyze the IL-4 and Ig E level and analysis was performed to determine the statistical significance of the relationship between the level of IL-4 and Ig E and Asthma.Results:1. No statistical significance was found on the relationship between Hp infection and Asthma(OR 0.76(95%CI=0.54-1.08) P=0.13,>0.05) using meta.2.The positive rate of Helicobacter pylori in the case group was 12.1%, and the positive rate of Helicobacter pylori in the healthy control group was 8.1%.No statistical significance was found on the relationship between Hp infection and Asthma using Chi-square test(P=0.2652,P >0.05).3. Using single factor chi-square test, statistical significance was found on factors including animal fur, dust mite, cold air, pollen, antibiotics treatment when children are 1-2years old, antibiotic treatment after children are 2 year old, habitat with smoke when children were born, habitat with smoke during the past three months, parents smoking around the time when children were born, humidity in children’s room, refusal to have pets and changing cleaning habits. Factors including how the children were born and how the children were fed do not have statistical significance on contributing to Asthma.Regressing all the above positive factors with Asthma show dust mite(OR=14.469,95%CI:1.660-126.082), pollen(OR=0.473,95%CI:3.786-28.974) 、 cold air(OR=5.963,95%CI:1.831-19.417), humidity in children’s room(OR=2.968,95%CI:1.004-8.773) and parents smoking(OR=1.843,95%CI:1.198-2.837) are independent factors contributing to Asthma, and dust mite, pollen are more dangerous while cold air, humidity in children’s room and parents smoking are less relevant.Antibiotic treatments when children are 1-2 years old(OR=0.457,95%CI:0.193-1.080) and antibiotic treatments when children are above 2 years old are protective factors for Asthma.Antibiotic treatments when children are above 2 years old works better than antibiotic treatments when children are 1-2 years old.4. IL-4 and Ig E are higher in the blood samples from children in the case group.IL-4(pg/ml) case group 141.0±124.5>comparison group 52.0595±64.4070,P=0.0006,<0.05; Ig E(ng/l) case group 33.2679±34.6853>comparison group17.5258±27.8485,P=0.0065,<0.05). IL-4 and Ig E are positively correlated(r=0.288,P<0.05). This indicates it is probably that Asthma progress through Ig E. Even though children in the recovery state do not have symptoms, they still have airway inflammatory cell infiltration and the airway is still very sensitive, indicting the possibility that the recurrence of Asthma is related to this.Conclusion:It is concluded that exposure to allergen including animal fur, dust mite, pollen and cold air as well as exposure to smoky environment when born may increase the risk of Asthma. Cesarean section and breast feeding for less than 6 months and Hp infection do not increase the risk of Asthma. The research also shows Asthma may develop through IL-4 and Ig E. |
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