Synthesis And Properties Of Bismuth Oxide/Sodium Alginate Hydrogel Composite Films

Objective:To prepare and evaluate the physical and chemical property,antimicrobialability, radiation protection, and cytocompatibility of bismuth oxide/sodium alginate hydrogel composite films, and provide experimental basis for hydrogel materialapplication in the field of radiation protection.Methods: The hydrogel films were prepared by chemical crosslinking with bismuth oxide and sodium alginate in different proportions(the mass ratio of bismuth oxide and sodium alginate were 0/1:6/1:3/2:3/1:1). The structure, morphology and mechanical properties of hydrogel films were studied by XRD, SEM, universal testing machine.The swelling rate and degradability of hydrogel films were studied by quantifying their quality change.The antibacterial property of hydrogel films was evaluated by S.aureus colony counting method.The radiation protection of films was studied by multichannel γ spectrometer system.The cytocompatibility of hydrogel films was determined by MTT method and fluorescence microscopy observations.Results:1) The XRD results showed that the characteristic crystal peak of bismuth oxide increased gradually with the increase of its content.SEM images showed that bismuth oxide evenly dispersed in porous hydrogel films.With the increase of bismuth oxide, the tensile strength increased first and then decreased, and the mass ratio of 6:1showed the highest tensile strength(4.6 MPa) in comparison to the others, elongation at break showed a trend of decline.The obtained swelling capacity for the hydrogel films was generally decreased by addition of bismuth oxide.The content of bismuth oxide had no statistical difference in degradation behavior.2) Antibacterial test suggested that the hydrogel films containing bismuth oxide had bacterial inhibitory abilities to S.aureus, and with increase of the content of bismuth oxide, the antibacterial activity also increased, the maximum inhibition rate was 55%.3) With the increase of the content of bismuth oxide, the γ-ray radiation protection increased, and the mass ratio of 1:1 was up to 15.3%.4) With the increase of the content of bismuth oxide, composite films had the effect on survival and growth of L929 cell, the cell survival rate decreased gradually, and the composite films of the mass ratio of 1:1 were co-cultured two days later, the cell survival rate was 80.5%. The cell morphology between the experimental group and control group was similarly observed by fluorescence microscope.Conclusion:The bismuth oxide/sodium alginate hydrogel composite films were prepared successfully, which exhibited antimicrobial properties and γ-ray radiation protection performance. The prepared composite hydrogel films have application prospects in the field of radiation protection.