Preparation Of New Type Hydrogel Films Possessing High Degree Of Swelling And Their Therapeutic Effect In Radiation Burn

Object: There are already many successful products in hydrogel dressings, and their effect in the clinical utilization is much fine. But the capability of the previous prepared hydrogel dressings absorbing wound surface effusion is generally inferior (the degree of swelling is relatively low), thus the hydrogel dressings need to be replaced frequently in the utilization. Our purpose is to investigate the materials and method of preparing hydrogel dressings possessing higher degree of swelling. Meanwhile, we manufactured the model of animal radiation burn and incorporated vascular endothelial growth factor (VEGF) into the prepared hydrogel dressings. Their therapeutic effect in radiation burn was investigated.Methods: Material A, material B, material C and material D. One or two materials among them were selected, singlly or mixedly dissolved in different concentration and ratio of quality, radiated crosslinking, to prepare hydrogel films. We observed the appearance, detected the degree of swelling, gel fraction, tensile strength of the prepared hydrogel films in different condition to look for the preparative method of ideal hydrogel film wound dressings. High-energy electron beam radiated the rat's haunch, the general radiative dose was 50Gy, to manufacture the animal model of radiation burn. We incorporated VEGF into the prepared hydrogel films, the control group were petrolatum gauze dressings, covered the burn surface respectively to carry out curing. And we measured the area of the wound surface ulcers, calculated healing time of the ulcers, take the specimens at different time, observed the pathomorphology changes of the radiation skin burn in the process of curing through HE staining.Results: After material A dissolved in concentration of 30% and 40%, the hydrogel films prepared possess better transparency, few bubbles, high degree of swelling, but the tensile strength is inferior. With the dose of the electron beam was 45kGy and the ratio of quality between material A and material B was 25:2, the hydrogels prepared possess maximal degree of swelling, higher transparency, better gel fraction and suitable tensile strength. However, with the ratio of quality was 25:6, the hydrogels prepared possess much ideal tensile strength, higher degree of swelling, better gel fraction and transparency. After incorporated VEGF into the hydrogel dressings, contrast petrolatum gauze dressings, the hydrogel dressings have satisfactory therapeutic effect in curing radiation burn.Conclusion:1. The hydrogels of material A possess better appearance character and high degree of swelling. The problem that the capability of the hydrogel dressings for clinic utilization absorbing wound surface effusion is inferior is solved, but the prepared hydrogel films have the shortage of inferior tensile strength.2. Material A and material B were mixed and dissolved, radiated crosslinking. The hydrogel films prepared respectively possess maximal degree of swelling and much ideal tensile strength. They can be used as new type hydrogel dressings possessing suitable function for the different wound in clinic.3. After incorporated VEGF into the prepared hydrogel film dressings, they have favourable effect in curing radiation burn.