Study On Nutritional Assessment Of Genetically Modified Crops “12-5” With NJB(Cry1Ab/ Cry2Aj)and G10evo Gene

Rencently, with the steadily increased global demand for corn, the planted area of transgenic plants expanded rapidly. Meanwhile food safety of gene modified corn gets more public attention. If inserting exogenous gene into conventional crops will cause changes in its nutrients, thus affecting the body’s absorption and utilization of nutrients, is the important content in safety assessment of transgenic corn.Objective To detect the difference on main content and in vivo digestibility in rats and pigs between genetically modified maize “12-5” and its parental “Ruifeng-1”. This study can give the transgenic corn “12-5” an nutritional assessment, and provide data support for the commercialize of this transgenic corn.Methods 1 The comparation on the compostion of GM corn “12-5”and its parental control Quality of protein/ fat/ carbohydrate/ fiber/ mineral/ vitamin/ moisture/ ash/ phytic acid in genetically modified maize and its parental maize was determined and compared with their paretal control, according to national standards. 2 Determine the effect of GM maize on the nutrients digestibiliy in rats Divided 80 Wistar rats randomly into four groups, every group was exposed continuous basic feed/GM maize feed/parental feed/casein feed separately for 14 days. Record the feed intake and weight; collect the feces; determine the quality of nutrients in feces and feed at last what contain protein/ fat/ carbohydrate/ calcium/ phosphorrus. Calculate the the apparent digestibility of these indexs above and the protein efficiency ratio/ the corrected protein efficiency ratio. 3 Study on the influence of GM maize “12-5” to the digestibility of nutrients in pigs Six castrated Du×chang×da pigs were fed with the GM maize feed, parental maize feed and the casein feed by turns according to the latin square design(One set with each two pigs). Collect the digesta through T-cannulas, and determine the quality of nutrients. Calculate the the apparent digestibility of protein/ amino acids/ fat/ fiber/ carbohydrate and the standard digetibility of amino acids.Results 1 There was no statistical difference between genetically modified maize “12-5” and its Parental maize “Ruifeng-1” on the quality of protein/ fat/ fiber/vitamin E/ potassium/ sodium and phytic acid(P>0.05). Ash quaility in GM maize “12-5” is higher than its parental control(P<0.05), and Carbohydrate is lower(P<0.05). 2 The apparent digestibility of protein/ fat/ carbohydrate/ calcium and protein efficiency ratio in GM group has no statistical diference with its parental group(P>0.05), but is higher than these two index in basic feed group(P<0.05) and phosphors in transgenic group is no lower than casein group(P>0.05), higher than the parental control group’s(P<0.05). 3 The apparent digestibility of protein/ fat/ fiber/ carbohydrate in pigs deserved to transgenic maize group has no statistical difference with pigs fed its paretal maize feed(P>0.05), and The apparent digestibility and the standard digetibility of 17 kinds of amino acids in pigs fed transgenic corn has not statistical difference between pigs fed its paretal maize(P>0.05).Conclusions 1 The quality of principal componet, ash exceptionally, of the transgenic corn “12-5” with the NJB(Cry1Ab/Cry2Aj) and G10 evo gene has no statistical difference with its parental maize “Ruifeng-1”. 2 The main nutrients’ digestibility in rats and pigs fed with transgenic corn “12-5” with the NJB(Cry1Ab/Cry2Aj) and G10 evo gene have no statistical difference with its parent corn “Ruifeng-1”. As a consequence of the above, the transgenic corn “12-5” with the NJB(Cry1Ab/ Cry2Aj) and G10 evo gene was nutritionally substantial-equivalent and its parent corn “Ruifeng-1”.