| Background and Objective: Bladder cancer is one of the most common malignant tumors in urology clinical, directly affects the survival of patients.Bladder cancer often begins in the change of the DNA, the majority research of bladder cancer etiology at present mainly focus on genetic changes, such as the activation of oncogene and tumor suppressor gene inactivation, but the basis biological of continuous proliferation of tumor cells mainly is the adjusting factors abnormally or disorderly which participate in the process of the regulation of cell cycle,that is its internal mechanism, and the molecular mechanism also has relation to the occurrence and development of bladder cancer.In addition, the information given in the cell cycle can reflect the tumor biological behavior characteristics and prognosis of the patients.This experiment mainly was to investigate the expression of p27 and MCM7 in bladder cancer, to explore the relationship with age, sex, tumor TNM staging and pathologic grading.Besides,we wanted to study the correlation of p27 and MCM7 expressed in bladder cancer, to look for new molecular markers associated with bladder cancer development, and to make for the diagnosis and treatment of bladder cancer and malignant behavior evaluation.Method: We collect 76 cases of bladder transitional cell carcinoma from the second hospital of tianjin medical university from January 2013 and October 2014,which were all confirmed by pathological examination and HE staining after surgery,and 18 cases of the normal or atypical hyperplasia bladder tissues were randomly selected as comparison, and all of the patients were not given radiotherapy and chemotherapy before operation which is basic requirement. Streptomyces avidin-peroxide enzyme immuneohistochemical method was adopted for testing the expression of p27 and MCM7 in all specimens.The experiment steps was according to kit which was purchased. All immunohistochemical result were observed by light microscope.SPSS17.0 software was used for statistical analysis, the pathological parameters(including age, sex, TNM staging, pathological classification) were analyzed by chi-square test.The Spearman rank correlation analysis was used to analyze the correlation of p27 and MCM7 expressed in bladder cancer tissues.Thestatistical significance was defined as P < 0.05.Results:(1) In bladder tissue,p27 and MCM7 expressed mainly in nuclei. In 76 cases of bladder cancer tissue,p27 positive expression rate was 40.8%(31/76) and MCM7 was 82.5%(62/76);In 18 cases of normal or atypical hyperplasia bladder tissues, p27 positive expression rate was 88.9%(16/18) and MCM7 was 11.1%(6/18).there were significant differences between p27 group and MCM7 group(χ2= 13.468, P < 0.05; χ2= 16.930, P < 0.05).(2) The expression of p27 and MCM7 had nothing to do with age and sex, but was associated with bladder cancer pathological grade and T stage(P < 0.05).(3) In 76 cases of bladder cancer tissues, the expression of p27 and MCM7 were commonly positive for 27 cases, with 10 cases of negative, Spearman correlation analysis indicated no obvious correlation(r = 0.118, P > 0.118).Conclusions:(1) the expression of p27 in bladder cancer tissues is significantly less than the normal or atypical hyperplasia bladder tissues, and MCM7 is higher than the latter, the differences between the experimental group and control group are significant, these results suggest that p27 and MCM7 may participate in the occurrence of bladder cancer.(2) the expression of p27 and MCM7 in bladder cancer tissues are closely related to the pathological grade and clinical T stage,so these results suggest that p27 and MCM7 may play a role in the invasion and metastasis of bladder cancer.(3) the expression of p27 and MCM7 in bladder cancer tissues have no significant correlation, but the combination of p27 and MCM7 in bladder cancer still be useful. |
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