Protective Effects Of Cyanidin-3-glucoside Nanoparticles Against UVB-induced Epidermal Damage In KM Mice Through The Regulation Of P53 Apoptosis

【Objective】 1. To establish an animal model of phototoxicity in UVB; 2. To investigate the effect of cyanidin-3-glucoside nanoparticles(Nano-C3G) and Cyanidin-3-Glucoside(C3G) on UVB-induced phototoxicity mice model; 3. To investigate the mechanism of Nano-C3 G and C3 G intervene the mitochondrial apoptosis pathway of p53.【Methods】 1. Appearance: observation evaluation; 2. Skin moisture and skin elasticity:Skin detection instrument to detect the skin moisture and skin elasticity of mice; 3. Pathology:Skin tissue hematoxylin eosin staining method; Immunohistochemical staining of TUNEL cells apoptosis; 4. Oxidative stress:The content of MDA and lipid peroxide were detected by the method of TBA; 5. DNA damage: Enzyme linked immunosorbent assay(ELISA) method for the detection of 8-hydroxy-desoxyguanosine(8-OHd G); 6. p53:Western blotting for p53 expression level; 7. Apoptosis associated proteins: a) Enzyme linked immunosorbent assay(ELISA) method for the detection of Bcl-2 family(Bax、Bcl-2); b) Western blotting for the expression level of Caspase family(Caspase-3、Caspase-9);【Results】1. Establish an animal model of phototoxicity with high dose UVB irradiating shaved Kunming mice, and then feeding for 24 hours; 2. Nano-C3G(125-500 u M)improve the appearance and the skin moisture of model mice; improve histologic appearance and the apoptotic index of TUNEL; and these effect of Nano-C3 G is stronger than C3 G under the same concentrations(500u M); 3. Nano-C3G(125-500 u M) can effectively reduce the LPO and MDA content induced by UVB(p<0.0001); 4. Nano-C3G(125-500 u M)can effectively reduce the 8-OHd G content induced by UVB(p<0.0001; and the effect of Nano-C3 G is stronger than C3 G under the same concentrations(500u M); 5. Nano-C3G(125-500 u M)can effectively downregulate p53 expression level(p<0.0001), balance Bcl-2 family(p<0.0001), ownregulate Caspase family(p<0.05) in UVB induced mice model of phototoxicity; and the effect of Nano-C3 G is stronger than C3 G under the same concentrations(500u M)(p<0.05);【Conclusion】 1. High does UVB induced apoptosis in mouse skin through the oxidative stress injury of photodynamic damage or the direct DNA injury of no-photodynamic damage; it upregulate p53 expression level through breaking the balance of Bcl-2 family, and then,eventually, activated Caspase family to start the apoptotic cascade; 2. Nano-C3G、C3G against UVB induced skin tissue cell apoptosis of mice, and the effect of Nano-C3 G is stronger than C3 G under the same concentrations; 3. Nano-C3G、C3G against UVB induced apoptosis through againsting the oxidative stress injury of photodynamic damage and the direct DNA injury of nophotodynamic damage; it downregulate p53 expression level through balance Bcl-2 family, then downregulate Caspase family to against the apoptotic cascade.