The Pharmaceutical Study On Anti Infection NZ Granula

Objective To establish a animal model suitable for compound NZ anti-infection in vivo studies.To clarify the anti-infection effects and the influence of the enzyme activity of immunity in animals of traditional Chinese medicine compound NZ. To get the information about the 5 multi-drug resistant strains ‘ sensitivity to traditional Chinese medicine compound NZ and other popular traditional Chinese medicine compound to fight infection on the market. Provide pharmic information for the development of traditional Chinese medicine new medicine about no cure or treatment effect is poorer for bacterial infectious diseases.Methods Compared the infection methods including injection, natural drinking water, medicine feeding etc.Based on the requirement of the research about traditional Chinese medicine compound NZ, Through the statistics of infection rates and survival rate, the observation of internal anatomical,the test of pathogen and other evaluation index.To establish a animal model which closer to the actual clinical infection. Base on the animal model, Symptoms,pathogenic test, the survival rate and the quality of survival can be taken as the indexes,Comprehensive assessment the treatment effects of this 15 different therapeutic schemes. Set test group and the control group respectively. Comparing three kinds of representative enzyme: muramidase,aspartate aminotransferase,acid phosphatase.And the transformation law within 96 h. Susceptivity to antibiotics of the bacteria was measured by K-B and agar diffusion methods. To observe in vitro bacteriostatic efficacy of traditional Chinese medicine compound NZ and other popular traditional Chinese medicine compound.Result 1prepare 5×105CUF/ml bacterium solution,the Infection animal model established by drinking water,which symptoms are most evident,mortality rate are6.7%, infection rate are 93.3%. 2 In the 15 dosage regimens, the number4,7,10,13,14 group were healed;the number 1,8,11 group were favor;the number2,5,12,15 group were progress;the number 3.6,9 and 16(control group) group were invalid.In the five well-healed project,The administration route of number 4 group are most convenient. That is to say,3.2mg/ml compound NZ solution, every 24 hours,for two consecutive times.3The transformation law of these three kinds of immunoenzyme within 96 has follows:1) Lysozyme(LZM):the level of experimental group achieve the optimal effect in 8h,and then gradually reduce, becomes flat at last. The control group activation of LZM is optimally in2 h,and then gradually reduce, becomes flat at last. from the beginning of 4h,showed significant difference between the two groups. The biggest difference in8 h,the missdistance reach 0.043U/ml. 2)Glutamic oxalacetic transaminase(AST/GOT): Traditional Chinese medicine compound NZ can help the cell normal secretion of AST,stimulate them survive,After 24 h,In contrast to the control group,the GOT activity of experimental group have significant difference(P<0.05)。After 48 h,the GOT activity of experimental group remain in the normal level.However,the GOT activity of control group markedly reduce. 3)Acid phosphatase(ACP):The level of ACP experimental group showed an increasing tendency between 4 and 12 h,gradually back to more normal levels after12h;The level of ACP control group showed a declined tendency between 4 and8 h,then approached to a flat and significantly lower than normal.After 8h,the activation of ACP between experimental group and control group showed remarkably different. At the time of 12 h,experimental group reached11.7UACP/ml,and the control group was only 6.5UACP/ml,ACP activity was44.4% lower.4 Traditional Chinese Medicine NZ effectiveness on inhibiting the growth of the five chosen types of bacteria.The diameter of bacteria inhibiting loop is larger than 15 mm,showed extremelysensitive. The minimum inhibitory concentration is 0.313~1.25mg/ml;The minimum bactericidal concentration is0.313~1.25mg/ml.The randomly selected 10 kinds of traditional Chinese medicine compound,which diameter of bacteria inhibiting loop are mainly concentrated below 10 mm,showed low-alcohol sensitive or insensitive.The MIC and MBC are mainly concentrated between 10 and 80mg/ml. The bacteriostatic activity of compound NZ and other experimental groups exists significant differences.Conclusion A strong repeatability,easy accessibility,high rates,closely nature infectious animal model is successfully established,It clear up the effect of anti-infection about compound NZ and preliminary dosage regimen was determined.In vivo immunization respect,compound NZ not merely can maintain the GOT activity and prevented the liver against the damage of hazardous substance but also promote enzymes activity of ACP and LZM, enhancing immunity. In additionbesides,compound NZ has the effectiveness on inhibiting the growth of 5 types ofdrug-resistant bacteria and have significant advantage over those herbal medicinal compound prescribe onsale. These results will provide experimental bases of new drugs development for cureless or poorer therapeutic effect of bacterial infection disease.