Develop A New Compound Herbal Medicine Gargle And Safety Evaluation

Objective By researching a variety of commonly used Chinese medicinal herb’s antibacterial effect,and selecting the composition of compound Chinese herbal medicine gargle and its suitable proportion,to develop a compound Chinese herbal medicine gargle can effectively inhibit the growth of Streptococcus mutans and Lactobacillus,and study it’santibacterial activity andtoxicology.Methods The filter paper diffusion method was used on each tested herbal extracts to test the bacteriostatic effect of the tested bacteria.According to the test results,the composition and formula of compound Chinese herbal medicine gargle were screened.The minimal inhibitory concentration was studied by minimal inhibitory concentration test.Through conducting acute toxicity,genetic toxicity and repeated vaginal mucosa irritation test,to study the toxicological properties of compound Chinese herbal medicine bacteriostatic gargle.Results Through the bacteriostasis experiment of 13 kinds of Chinese herbal medicine,bacteriostatic ring experiment results showed that Scutellaria baicalensis Georgi and Cortex Phellodendri had the best inhibitory effect on Streptococcus mutans and Lactobacillus.According to the selection of Chinese herbal medicine type as simple as possible,radix scutellariae and cortex phellodendri were selected as components of the compound Chinese herbal medicine gargle.The minimum inhibitory concentration of scutellaria baicalensis and cortex phellodendri chinensis on two kinds of caries bacteria was studied.The results showed that the minimum inhibitory concentration of scutellaria baicalensis and cortex phellodendri was 125mg/ml and 125mg/ml,respectively.The minimum inhibitory concentration of scutellaria baicalensis Georgi and cortex phellodendri was250mg/ml and 25.25mg/ml,respectively.The two kinds of Chinese herbal medicine were tested in different proportions and the results showed that the formula of compound Chinese herbal medicine gargle was 8:1.The amount of Scutellaria baicalensis Georgi and Cortex Phellodendri were 62.5mg/ml and 7.825mg/ml respectively among them,and the minimum inhibitory concentration was 62.5mg/ml and7.825mg/ml.The stability of the compound Chinese herbal medicine gargle your mouth was tested.the results showed that the minimum inhibitory concentration of the rinse your mouth solution unchanged,the effective bacteriostatic effect did not change,and had good stability,reached the 2002 edition disinfection technical specification.The results of an acute toxicity test of the new compound Chinese herbal medicine bacteriostatic gargle showed LD50 was greater than 5000 mg/(kg·BW),and the gargle belong to the actual non-toxic substances;Micronucleus test results showed there was no significant difference between the test groups and the negative control group(P>0.05),compared with the positive control group the difference was significant(P<0.05),and there is no no damage effect on in vivo chromosome;Multiple vaginal mucosa irritation test was carried out and no vaginal congestion,edema and erosion were found.,the compound Chinese herbal medicine gargle has no mucosa irritation and can be used safely.Conclusion The new compound Chinese herbal medicine gargle’s dispensation is Scutellaria baicalensis 62.5mg/ml and Cortex Phellodendri chinensis 7.825mg/ml.The effective antimicrobial content was baicalin 7.875 mg/ml,and berberine hydrochloride0.336 mg/ml,can inhibit the growth of Streptococcus mutans and lactobacillus,has no genetic toxicity and mucosal irritation,belong to the actual non-toxic substances and can be used safely;the bacteriostatic effect and stability of the compound Chinese herbal medicine bacte-r iostatic gargle reached 2002 edition "disinfection technical specifications" requirement.