The Research On Protective Effects Of Coldronate-liposome On Severe Acute Pancreatitis Complicated With Cerebral Damage In Rats

Objective:The aim of this study was to study the influence of coldronate-liposome on the brain of rats with severe acute pancreatitis(SAP), and to investigate the protective effects of coldronate-liposome on cerebral damage caused by severe acute pancreatitis in rats. Methods:Coldronate-liposome was prepared through the means of thin film. The models of SAP were performed by prinking injection of 5% sodium taurocholate into the pancreatic capsule. A total of 48 male healthy SD rats were randomly divided into the sham group(Sham), SAP+liposome group(LB), SAP+ Coldronate-liposome group(LC). LB group were injected with liposome(4 m L / kg) while LC group were injected with clodronate liposomes(4 m L / kg) in tail vein immediately after the SAP model performed. The change of amylase(AMS) in serum and the level of IL-6, TNF-α were obtained at 2 h and 6 h after operation respectively. Pathologic alteration of pancreas was observed and pathological score of pancreatic tissue was also estimated. Pathologic alteration of brain tissue was observed as well. The water and Evan’s blue content of brain tissues, the content of MBP in cerebrospinal fluid were detected. Results:Compared with Sham group, serum levels of AMS were significantly elevated in group LB(P<0.01). In LC group, serum levels of AMS were significantly decreased compared with LB group(P<0.01).Compared with Sham group, the serum levels of IL-6, TNF-α were significant elevated at 2 and 6 hours in LB group(P<0.01). In LC group, the serum levels of IL-6, TNF-α were significant decreased at 2 and 6 hours compared with LB group(P<0.01). Compared with Sham group, the pancreatic tissue pathological score were significant elevated at 2 and 6 hours in LB group(P<0.01). In LC group, the pancreatic tissue pathological score were significant decreased at 2 and 6 hours compared with LB group( P<0.01).Compared with the sham group, the pathological injury of brain tissue was significantly increased at each time in LB group. In LC group, the pathological injury of brain tissue was significant decreased at each time compared with LB group.Compared with Sham group, the water and Evan’s blue content of brain tissues, the content of MBP in cerebrospinal fluid were significant elevated at 2 and 6 hours in LB group(P<0.01). In LC group, the water and Evan’s blue content of brain tissues, the content of MBP in cerebrospinal fluid were significant decreased at 2 and 6 hours compared with LB group(P<0.01). Conclusion:Macrophages plays an integral role in SAP complicated with multiple organ failure, Monocytes/macrophages can be depleted via phagocytosis of liposome encapsulated coldronate, and reduce the release of inflammatory mediators. Coldronate-liposome plays a protective role on cerebral damage caused by SAP in rats.